PXD023415

PXD023415 is an original dataset announced via ProteomeXchange.

Title	The nucleolus functions as the compartment for histone 1 H2B protein degradation
Description	Histones are the main components in chromatin organization, and the protein levels of histones significantly affect chromatin assembly. However, how the protein levels of histones are regulated, especially whether and how histones are degraded, is largely unclear. Here, we found that histone H2B is mainly degraded through the proteasome mediated pathway, and the lysine 120 site of H2B is essential for the K48-linked polyubiquitination and protein degradation of H2B. Moreover, our results further indicated that the degradation-impaired H2BK120R mutant shows an increased nucleolus localization, and inhibition of the proteasome results in an elevated nucleolus distribution of wild-type H2B, which is similar to that of H2BK120R mutants. More importantly, our in vitro data showed that only the nucleolus fractions can ubiquitinate and degrade the purified H2B, suggesting that the nucleolus, in addition to its canonical roles in the regulation of ribosome genesis and protein translation, is likely associated with H2B protein degradation. Therefore, these findings revealed a novel mechanism for the regulation of H2B protein degradation in which a nucleolus associated proteasome pathway is involved.
HostingRepository	PRIDE
AnnounceDate	2021-09-09
AnnouncementXML	Submission_2021-09-09_15:54:29.030.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	sheng li
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	No PTMs are included in the dataset
Instrument	timsTOF Pro

Revision	Datetime	Status	ChangeLog Entry
0	2021-01-05 01:34:17	ID requested
⏵ 1	2021-09-09 15:54:29	announced

Liu Y, Wang Y, Yang L, Sun F, Li S, Wang Y, Zhang GA, Dong T, Zhang LL, Duan W, Zhang X, Cui W, Chen S, The nucleolus functions as the compartment for histone H2B protein degradation. iScience, 24(4):102256(2021) [pubmed]

submitter keyword: H2B

protein degradation

nucleolus

polyubiquitination

Su chen
contact affiliation	Xi’an Jiao Tong University Health Science Center
contact email	chensu@xjtu.edu.cn
lab head
sheng li
contact affiliation	Jining Medical University
contact email	lishengjnmc@163.com
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2021/09/PXD023415

PRIDE project URI

• PRIDE
  1. PXD023415
     1. Label: PRIDE project
     2. Name: The nucleolus functions as the compartment for histone 1 H2B protein degradation