PXD023404

PXD023404 is an original dataset announced via ProteomeXchange.

Title	Dictyostelium DIA LFQ proteomics of vegetative and developmental growth
Description	Dictyostelium discoideum behavior depends on nutrients. When sufficient food is present these amoebae exist in a unicellular state (vegetative growth), but upon starvation they aggregate into a multicellular organism (developmental growth). For proteomics 30 minutes and eight hour cultures of D. discoideum under vegetative and starved growth conditions were compared by DIA-LFQ. This unique biology makes D. discoideum an ideal model for investigating how fundamental metabolism commands cell differentiation and function. We show here that reactive oxygen species (ROS), generated as a consequence of nutrient limitation, lead to the sequestration of the amino acid cysteine in the antioxidant glutathione, limiting the use of its sulfur atom for processes such as protein translation and FeS cluster-containing enzyme activity that contribute to mitochondrial metabolism and cellular proliferation. Such regulated sulfur sequestration maintains D. discoideum in a non-proliferating state that paves the way for multicellular development. This new mechanism of ROS signaling highlights oxygen and sulfur as simple, early evolutionary signaling molecules dictating cell fate, with implications for responses to nutrient fluctuations in higher eukaryotes.
HostingRepository	PRIDE
AnnounceDate	2021-03-23
AnnouncementXML	Submission_2021-03-22_23:49:46.505.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Gerhard Mittler
SpeciesList	scientific name: Dictyostelium discoideum (Slime mold); NCBI TaxID: 44689;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue; deamidated residue
Instrument	Q Exactive Plus

Revision	Datetime	Status	ChangeLog Entry
0	2021-01-04 05:23:46	ID requested
⏵ 1	2021-03-22 23:49:47	announced

Kelly B, Carrizo GE, Edwards-Hicks J, Sanin DE, Stanczak MA, Priesnitz C, Flachsmann LJ, Curtis JD, Mittler G, Musa Y, Becker T, Buescher JM, Pearce EL, Sulfur sequestration promotes multicellularity during nutrient limitation. Nature, 591(7850):471-476(2021) [pubmed]

submitter keyword: Dictyostelium discoideum

non-starved (vegetative)

starved (developmental)

DIA hyper reaction monitoring

Spectronaut

sulfur metabolism

reactive oxygen species;

Gerhard Mittler
contact affiliation	Proteomics Unit Max Planck Institute of Immunobiology and Epigenetics Stuebeweg 51 79108 Freiburg, Germany
contact email	mittler@ie-freiburg.mpg.de
lab head
Gerhard Mittler
contact affiliation	Proteomics Unit, Max Planck Institute of Immunobiology and Epigenetics
contact email	mittler@ie-freiburg.mpg.de
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2021/03/PXD023404

PRIDE project URI

• PRIDE
  1. PXD023404
     1. Label: PRIDE project
     2. Name: Dictyostelium DIA LFQ proteomics of vegetative and developmental growth