PXD023379

PXD023379 is an original dataset announced via ProteomeXchange.

Title	N-Glycome and N-Glycoproteome of a Hematophagous Parasitic Nematode Haemonchus
Description	N-glycosylation is a physiologically vital post-translational modification of proteins in eukaryotic organisms. Initial work on Haemonchus contortus – an economically-important blood-sucking nematode of ruminants with a broad geographical distribution – has shown that the parasite harbors N-glycans with exclusive core chitobiose modifications, and severel immunogenic proteins (e.g., amino- and metallo-peptidases) in the adult worms are N-glycosylated. However, an informative atlas of N-glycosylation in H. contortus is not yet available. Herein, we report a total of 291 N-glycosylated proteins with 425 sites in this parasite. Functional analyses of glycoproteome revealed significant enrichment of the peptidase families (e.g., peptidase C1 and M1), many of which are potential vaccine targets. Besides, the glycan-rich conjugates are distributed primarily in the intestine and gonads of the adult worms. These data, taken together, provide a comprehensive insight into the N-glycosylation of a prevalent parasitic nematode, while underlining its significance for the infection and prophylaxis.
HostingRepository	PRIDE
AnnounceDate	2021-09-09
AnnouncementXML	Submission_2021-09-09_15:47:21.676.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Chunqun Wang
SpeciesList	scientific name: Haemonchus contortus; NCBI TaxID: 6289;
ModificationList	complex glycosylation
Instrument	Orbitrap Fusion

Revision	Datetime	Status	ChangeLog Entry
0	2021-01-04 03:10:38	ID requested
⏵ 1	2021-09-09 15:47:22	announced

Wang C, Gao W, Yan S, Zhu XQ, Suo X, Liu X, Gupta N, Hu M, . Comput Struct Biotechnol J, 19():2486-2496(2021) [pubmed]

submitter keyword: Haemonchus contortus, N-glycosylation, N-glycan, mass spectrometry, glycopeptide

Min Hu
contact affiliation	State Key Laboratory of Agricultural Microbiology, College of Veterinary Medicine, Huazhong Agricultural University
contact email	mhu@mail.hzau.edu.cn
lab head
Chunqun Wang
contact affiliation	State Key Laboratory of Agricultural Microbiology, College of Veterinary Medicine, Huazhong Agricultural University
contact email	wangchunqun@webmail.hzau.edu.cn
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD023379
     1. Label: PRIDE project
     2. Name: N-Glycome and N-Glycoproteome of a Hematophagous Parasitic Nematode Haemonchus