PXD023362

PXD023362 is an original dataset announced via ProteomeXchange.

Title	Identification of intracellular glycosaminoglycan-interacting proteins by affinity purification mass spectrometry
Description	Glycosaminoglycans (GAGs) are essential functional components of the extracellular matrix (ECM). Artificial GAGs like sulfated hyaluronan (sHA) exhibit pro-osteogenic properties and boost healing processes and hence are of high interest for supporting wound healing. Although sulfated GAGs (sGAGs) are internalized into cells, knowledge of intracellular effects and interaction partners is scarce. Here we used an affinity-purification mass spectrometry-based (AP-MS) approach to identify novel and particularly intracellular sGAG-interacting proteins from human bone marrow stromal cells (hBMSC). Overall, 478 proteins were found interacting with at least one of four distinct sGAGs. Enrichment analysis for protein localization showed that mainly intracellular and cell-associated interacting proteins were identified. Only a limited fraction of about 10 % was annotated for extracellular regions. The interactions of the 2-macroglobulin receptor-associated protein (LRPAP1), exportin-1 (XPO1), and Serine protease HTRA1 (HTRA1) were confirmed in reverse assays.
HostingRepository	PRIDE
AnnounceDate	2021-11-03
AnnouncementXML	Submission_2021-11-03_07:14:58.388.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Henning Großkopf
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2021-01-04 01:48:48	ID requested
⏵ 1	2021-11-03 07:14:59	announced

Gro, ß, kopf H, Vogel S, M, ü, ller CD, K, ö, hling S, D, ü, rig JN, M, ö, ller S, Schnabelrauch M, Rademann J, Hempel U, von Bergen M, Schubert K, Identification of intracellular glycosaminoglycan-interacting proteins by affinity purification mass spectrometry. Biol Chem, 402(11):1427-1440(2021) [pubmed]

submitter keyword: biotin-GAG, hBMSC, LC-MS/MS, pull-down approach, SaOS2/osteoblast cell line, sulfated glycosaminoglycan derivatives

Martin von Bergen
contact affiliation	Department of Molecular Systems Biology, Helmholtz Centre for Environmental Research – UFZ, Leipzig, Germany
contact email	martin.vonbergen@ufz.de
lab head
Henning Großkopf
contact affiliation	Molecular Systems Biology Helmholtz Centre for Environmental Research GmbH - UFZ Leipzig, Germany
contact email	henning.grosskopf@ufz.de
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2021/11/PXD023362

PRIDE project URI

• PRIDE
  1. PXD023362
     1. Label: PRIDE project
     2. Name: Identification of intracellular glycosaminoglycan-interacting proteins by affinity purification mass spectrometry