PXD023358

PXD023358 is an original dataset announced via ProteomeXchange.

Title	Comprehensive Analysis of Tryptic Peptides Arising from Disulfide Linkages in NISTmAb
Description	This work presents methods for identifying and then creating a mass spectral library for disulfide-linked peptides originating from the NISTmAb, a reference material of humanized IgG1k monoclonal antibody (RM 8671). Analyses involved both partially-reduced and non-reduced samples under neutral and weakly basic conditions, followed by nanoflow liquid chromatography tandem mass spectrometry (LC-MS/MS).
HostingRepository	PRIDE
AnnounceDate	2021-02-08
AnnouncementXML	Submission_2022-01-13_04:00:33.518.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Qian Dong
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	disulfide crosslinked residues
Instrument	Orbitrap Fusion Lumos; Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2021-01-04 01:30:21	ID requested
1	2021-02-08 11:57:19	announced
⏵ 2	2022-01-13 04:00:34	announced	2022-01-13: Updated publication reference for PubMed record(s): 33555887.

Dong Q, Yan X, Liang Y, Markey SP, Sheetlin SL, Remoroza CA, Wallace WE, Stein SE, Comprehensive Analysis of Tryptic Peptides Arising from Disulfide Linkages in NISTmAb and Their Use for Developing a Mass Spectral Library. J Proteome Res, 20(3):1612-1629(2021) [pubmed]

submitter keyword: cysteine, disulfide, disulfide bond, disulfide-linked peptides, NISTmAb, mass spectral library

Stephen E. Stein
contact affiliation	Biomolecular Measurement Division, National Institute of Standards and Technology (NIST)
contact email	stephen.stein@nist.gov
lab head
Qian Dong
contact affiliation	NIST
contact email	qiand3161@gmail.com
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD023358
     1. Label: PRIDE project
     2. Name: Comprehensive Analysis of Tryptic Peptides Arising from Disulfide Linkages in NISTmAb