PXD023347

PXD023347 is an original dataset announced via ProteomeXchange.

Title	USP36-mediated deubiquitination of DOCK4 contributes to the diabetic renal tubular epithelial cell injury via Wnt/β-catenin signaling pathway
Description	Diabetic kidney disease (DKD) has become the leading cause of end-stage renal diseases, but the efficacy of current treatment remains unsatisfactory. The pathogenesis of DKD needs a more in-depth research. Ubiquitin specific proteases 36 (USP36), a member of deubiquitinating enzymes family, has aroused wide concerns for its role in deubiquitinating and stabilizing target proteins. Nevertheless, the role of USP36 in diabetes has never been reported yet. Herein, we identified an increased expression of USP36 both in vitro and in vivo in diabetic renal tubular epithelial cells (TECs), and its overexpression is related to the enhanced epithelial-to-mesenchymal transition (EMT). Further investigation into the mechanisms proved that USP36 could directly bind to and mediate the deubiquitination of dedicator of cytokinesis 4 (DOCK4), a guanine nucleotide exchange factor (GEF) that could activate Wnt/β-catenin signaling pathway and induce EMT. Our study revealed a new mechanism that USP36 participates in the pathogenesis of DKD, and provided potential intervening targets accordingly.
HostingRepository	PRIDE
AnnounceDate	2021-09-09
AnnouncementXML	Submission_2021-09-09_15:45:04.832.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Suwei Zhu
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	TMT6plex-126 reporter+balance reagent acylated residue
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2021-01-04 00:19:45	ID requested
⏵ 1	2021-09-09 15:45:05	announced

Zhu S, Hou S, Lu Y, Sheng W, Cui Z, Dong T, Feng H, Wan Q, -Catenin Signaling Pathway. Front Cell Dev Biol, 9():638477(2021) [pubmed]

submitter keyword: ubiquitin specific proteases 36, dedicator of cytokinesis 4, Wnt/β-catenin, diabetic kidney disease, deubiquitination, epithelial-to-mesenchymal transition

Suwei Zhu
contact affiliation	Shandong University
contact email	1446245184@qq.com
lab head
Suwei Zhu
contact affiliation	Shandong Uniersity
contact email	zhangzheyu@aksomics.com
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD023347
     1. Label: PRIDE project
     2. Name: USP36-mediated deubiquitination of DOCK4 contributes to the diabetic renal tubular epithelial cell injury via Wnt/β-catenin signaling pathway