PXD023346

PXD023346 is an original dataset announced via ProteomeXchange.

Title	N- and O-glycosylation patterns of the SARS-CoV-2 spike protein reveal a new “O- follow-N” rule
Description	Severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2), the causative agent of COVID-19, has caused a worldwide pandemic since its discovery at the end of 2019. The spike (S) protein of SARS-CoV-2 is responsible for the host cell entry and the critical targets of the immune system. Consequently, S protein has been extensively used in antibody and vaccine studies. Here, we identified a total of 23 N-glycosites, including N334 in the noncanonical consensus motif N-X-C, on the full-length wild-type S protein protomer. Furthermore, 55 O-glycosylation sites were identified, and this uncovered a pattern of O-glycosylation near N-glycosites, which we named the “O-follow-N” rule, revealed by site-specific mutagenesis. The comprehensive mapping of the S protein O- and N-glycosylation landscape provides further information for studying the mechanisms underlying viral pathogenesis and immune evasion.
HostingRepository	iProX
AnnounceDate	2021-03-24
AnnouncementXML	Submission_2023-02-20_22:58:00.995.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Shuaixin Gao
SpeciesList	scientific name: Severe acute respiratory syndrome coronavirus 2; NCBI TaxID: 2697049;
ModificationList	N-glycosylated residue; O-glycosyl-L-serine; O-glycosyl-L-threonine
Instrument	Orbitrap Eclipse

Revision	Datetime	Status	ChangeLog Entry
0	2021-01-04 00:19:13	ID requested
1	2021-01-04 00:22:49	announced
⏵ 2	2023-02-20 22:58:01	announced	2023-02-21: Update information.

Tian W, Li D, Zhang N, Bai G, Yuan K, Xiao H, Gao F, Chen Y, Wong CCL, Gao GF, O-glycosylation pattern of the SARS-CoV-2 spike protein reveals an "O-Follow-N" rule. Cell Res, 31(10):1123-1125(2021) [pubmed]

submitter keyword: SARS-CoV-2, spike protein,O-glycosylation sites,O- follow-N

Catherine CL Wong
contact affiliation	Center for Precision Medicine Multi-Omics Research, Peking University Health Science Center, Peking University
contact email	catherine_wong@bjmu.edu.cn
lab head
Shuaixin Gao
contact affiliation	Center for Precision Medicine Multi-Omics Research, Peking University Health Science Center, Peking University
contact email	gaoshuaixin@126.com
dataset submitter

iProX dataset URI