PXD023341

PXD023341 is an original dataset announced via ProteomeXchange.

Title	Proteomic identification of recombinant nucleocapsid N protein 40 kDa fragment from SARS-CoV-2
Description	Serological testing is important methodfor diagnosis of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infection. Nucleocapsid (N) protein is the most abundant virus derived protein and strong immunogen. We aimed to find its efficient, low-cost production. SARS-CoV-2 recombinant fragment of nucleocapsid protein (rfNP; 58-419 aa) was expressed in E. coli in soluble form, purified and characterized biochemically and immunologically. Purified rfNP has secondary structure of full-length recombinant N protein, with high percentage of disordered structure (34.2 %) and of β-sheet (40.7 %). rfNP was tested in immunoblot using sera of COVID-19 convalescent patients. ELISA was optimized with sera of RT-PCR confirmed positive symptomatic patients and healthy individuals. IgG detection sensitivity was 96% (47/50) and specificity 97% (67/68), while IgM detection was slightly lower (94% and 96.5%, respectively). Cost-effective approach for soluble recombinant N protein fragment production was developed, with reliable IgG and IgM antibodies detection of SARS-CoV-2 infection.
HostingRepository	PRIDE
AnnounceDate	2021-02-17
AnnouncementXML	Submission_2021-02-16_22:42:04.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD023341
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Katarina Smiljanic
SpeciesList	scientific name: SARS coronavirus Sin3765V; NCBI TaxID: 267386;
ModificationList	carbamoylated residue; dehydrated residue; acetylated residue; monohydroxylated residue; monosodium salt; iodoacetamide derivatized residue; deamidated residue
Instrument	LTQ Orbitrap XL

Revision	Datetime	Status	ChangeLog Entry
0	2021-01-04 00:04:46	ID requested
⏵ 1	2021-02-16 22:42:04	announced

Djukic T, Mladenovic M, Stanic-Vucinic D, Radosavljevic J, Smiljanic K, Sabljic L, Devic M, Cujic D, Vasovic T, Simovic A, Radomirovic M, Cirkovic Velickovic T, Expression, purification and immunological characterization of recombinant nucleocapsid protein fragment from SARS-CoV-2. Virology, 557():15-22(2021) [pubmed]

ProteomeXchange project tag: Sars-cov-2, Covid-19

submitter keyword: Recombinant nucleocapsid protein, N protein fragment, covid-19, SARS-CoV-2, prokaryotic expression;serological assay

Tanja D. Ćirković Velicković
contact affiliation	Tanja Ćirković Velicković, PhD Professor of Biochemistry & Corresponding member of Serbian Academy of Sciences and Arts University of Belgrade-Faculty of Chemistry, Center of Excellence for Molecular Food Sciences and Department of Biochemistry, Studentski trg 12-16, 11 000 Belgrade, Serbia. Professor of Food Chemistry Department of Environmental Technology, Food Technology and Molecular Biotechnology, Ghent University Global Campus, Yeonsu-Gu, 21985 Incheon, Republic of Korea
contact email	tcirkov@chem.bg.ac.rs
lab head
Katarina Smiljanic
contact affiliation	Senior Research Associate at University of Belgrade-Faculty of Chemistry, Center of Excellence for Molecular Food Sciences
contact email	katarinas@chem.bg.ac.rs
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD023341
     1. Label: PRIDE project
     2. Name: Proteomic identification of recombinant nucleocapsid N protein 40 kDa fragment from SARS-CoV-2