PXD023338

PXD023338 is an original dataset announced via ProteomeXchange.

Title	Blocking utilization of major plant biomass polysaccharides leads Aspergillus niger towards utilization of minor components.
Description	Fungi produce a wide range of enzymes that allow them to grow on diverse plant biomass. Wheat bran is a low-cost substrate with high potential for biotechnological applications. It mainly contains cellulose and (arabino)xylan, with starch, proteins, lipids and lignin as minor components. In this study, we dissected the regulatory network governing wheat bran degradation in Aspergillus niger. Deletion of genes encoding transcription factors involved in (hemi-)cellulose utilization (XlnR, AraR, ClrA and ClrB) individually and in combination significantly reduced production of polysaccharide-degrading enzymes, but retained substantial growth on wheat bran. Proteomic analysis suggested the ability of A. niger to grow on minor carbon components, such as starch, which was confirmed by the additional deletion of the amylolytic regulator AmyR. Growth was further reduced but not impaired, indicating that other minor components provide sufficient energy for residual growth, displaying the flexibility of A. niger, and likely other fungi, in carbon utilization.
HostingRepository	PRIDE
AnnounceDate	2021-06-28
AnnouncementXML	Submission_2021-06-28_06:59:20.232.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD023338
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Marcos Di Falco
SpeciesList	scientific name: Aspergillus niger NRRL3; NCBI TaxID: 1451139;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue
Instrument	LTQ Orbitrap Velos

Revision	Datetime	Status	ChangeLog Entry
0	2021-01-03 23:49:18	ID requested
⏵ 1	2021-06-28 06:59:20	announced

Kun RS, Garrigues S, Di Falco M, Tsang A, de Vries RP, Blocking utilization of major plant biomass polysaccharides leads Aspergillus niger towards utilization of minor components. Microb Biotechnol, 14(4):1683-1698(2021) [pubmed]

submitter keyword: Aspergillus niger, combinatorial deletions, plant biomass, regulation, transcription factors, LC-MSMS.

Ronald de Vries
contact affiliation	Group Leader Fungal Physiology Professor in Fungal Molecular Physiology at Utrecht University, The Netherlands
contact email	r.devries@wi.knaw.nl
lab head
Marcos Di Falco
contact affiliation	Concordia University
contact email	marcos.difalco@concordia.ca
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD023338
     1. Label: PRIDE project
     2. Name: Blocking utilization of major plant biomass polysaccharides leads Aspergillus niger towards utilization of minor components.