PXD023336

PXD023336 is an original dataset announced via ProteomeXchange.

Title	Protein kinase C activation drives a differentiation program in an oligodendroglial precursor model through the modulation of specific biological networks
Description	Protein kinase C (PKC) activation induces cellular reprogramming and differentiation in a variety of cellular models. Although many effectors of PKC physiological actions have been elucidated, the molecular mechanisms that regulate oligodendrocytes differentiation after PKC activation are still unclear. Here we applied a liquid chromatography - mass spectrometry (LC-MS/MS) approach to identify proteins, and biological processes, modulated after PKC activation in the established PKC-induced differentiation model, MO3.13 oligodendroglial cell line. Our analysis revealed that PKC signaling activation leads to the down-regulation of biological processes related to cell cycle and DNA replication and the up-regulation of a glial like developmental program. Our data suggested a role for ROCK in the modulation of MO3.13 phenotype, cytoskeletal and mechanotransduction dynamics but not in the modulation of cell cycle and calcium transport. Overall, our findings provides a resource for elucidating the PKC-mediated signaling network contributing to a more robust knowledge of the molecular dynamics leading to this cell fate transition.
HostingRepository	PRIDE
AnnounceDate	2021-09-09
AnnouncementXML	Submission_2021-09-09_15:43:40.929.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Marina Damato
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2021-01-03 23:25:54	ID requested
⏵ 1	2021-09-09 15:43:41	announced

Damato M, Cardon T, Wisztorski M, Fournier I, Pieragostino D, Cicalini I, Salzet M, Vergara D, Maffia M, Protein Kinase C Activation Drives a Differentiation Program in an Oligodendroglial Precursor Model through the Modulation of Specific Biological Networks. Int J Mol Sci, 22(10):(2021) [pubmed]

submitter keyword: PKC, differentiation, oligodendrocytes, signaling, mass spectrometry

Michel Salzet (Lab Head)
contact affiliation	PRISM - Unité Inserm 1192 Université de Lille France
contact email	michel.salzet@univ-lille.fr
lab head
Marina Damato
contact affiliation	University of Salento
contact email	marina.damato@hotmail.it
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD023336
     1. Label: PRIDE project
     2. Name: Protein kinase C activation drives a differentiation program in an oligodendroglial precursor model through the modulation of specific biological networks