<<< Full experiment listing

PXD023334-1

PXD023334 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleComparison of unit resolution versus high-resolution accurate mass for parallel reaction monitoring
DescriptionParallel reaction monitoring (PRM) is an increasingly popular alternative to selected reaction monitoring (SRM) for targeted proteomics. PRM’s strengths over SRM are that it monitors all product ions in a single spectrum, thus eliminating the need to select interference-free product ions prior to data acquisition, and that it is most frequently performed on high-resolution instruments, such as quadrupole-orbitrap and quadrupole-time of flight instruments. Here, we show that the primary advantage of PRM is the ability to monitor all transitions in parallel, and that high-resolution data are not necessary to obtain high quality quantitative data. We run the same scheduled PRM assay, measuring 432 peptides from 126 plasma proteins, multiple times on a Orbitrap Eclipse Tribrid mass spectrometer, alternating separate liquid chromatography-tandem mass spectrometry runs between the high resolution Orbitrap and the unit resolution linear ion trap for PRM. We find that both mass analyzers have similar technical precision, and that the linear ion trap’s superior sensitivity gives it better lower limits of quantitation on over 62% of peptides in the assay.
HostingRepositoryPanoramaPublic
AnnounceDate2021-05-06
AnnouncementXMLSubmission_2021-05-06_09:38:10.990.xml
DigitalObjectIdentifier
ReviewLevelNon peer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportSupported dataset by repository
PrimarySubmitterLilian Heil
SpeciesList scientific name: Homo sapiens; NCBI TaxID: 9606;
ModificationListCarbamidomethyl; Oxidation; Label:13C(6)15N(2); Label:13C(6)15N(4)
InstrumentOrbitrap Eclipse; Orbitrap Fusion Lumos
Dataset History
RevisionDatetimeStatusChangeLog Entry
02021-01-02 11:51:35ID requested
12021-05-06 09:38:11announced
22021-08-19 08:58:20announced: Added PubMed Id
Publication List
Heil LR, Remes PM, MacCoss MJ. Comparison of unit resolution versus high-resolution accurate mass for parallel reaction monitoring. bioRxiv [Internet]. 2021 Jan 1;2021.05.04.442680. Available from: http://biorxiv.org/content/early/2021/05/04/2021.05.04.442680.abstract
Keyword List
submitter keyword: mass spectrometry, limit of quantitation, precision, PRM
Contact List
Michael MacCoss
contact affiliationUniversity of Washington
contact emailmaccoss@uw.edu
lab head
Lilian Heil
contact affiliationUniversity of Washington
contact emaillheil2@uw.edu
dataset submitter
Full Dataset Link List
Panorama Public dataset URI