PXD023315

PXD023315 is an original dataset announced via ProteomeXchange.

Title	Proteomic Analysis of Xinjiang Black Bee's Royal Jelly Using the Osboren Classification Method
Description	Royal jelly has long been recognized as health food, with a high content of proteins. These proteins play important roles in honeybee caste and human health, but the proteomic analysis of those low-abundance proteins in royal jelly is always a challenge. Herein, we used the Osboren classification method to separate the royal jelly proteins of Xinjiang black bees, a sub-species of Apis mellifera mellifera, into various fractions. The globulin, ethanol-soluble protein and glutelin fractions were further separated by SDS-PAGE, and proteomic analysis was carried out by LC-MS/MS and searching against the NCBI database. A total of 63 proteins with definitive names were identified, in which 41 proteins were identified for the first time in royal jelly. The Osboren classification method combined with one-dimensional gel electrophoresis based proteomic analysis allows the identification of low-abundance proteins, and greatly extends the knowledge about the components and functions of royal jelly proteins.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_06:53:36.444.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Long Chen
SpeciesList	scientific name: Apis mellifera (Honeybee); NCBI TaxID: 7460;
ModificationList	No PTMs are included in the dataset
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2020-12-25 03:23:42	ID requested
1	2024-08-09 02:55:53	announced
⏵ 2	2024-10-22 06:53:38	announced	2024-10-22: Updated project metadata.

Pei P, Guo W, Qi M, Jiang J, Feng S, Luo SZ, Chen L, Identification of low-abundance proteins in the royal jelly using the Osborne classification method. J Proteomics, 306():105266(2024) [pubmed]

10.1016/j.jprot.2024.105266;

submitter keyword: oyal jelly

proteome

Osboren classification

low-abundance protein

Long Chen
contact affiliation	College of Life Science & Technology Beijing University of Chemical Technology Beijing, 10029, China
contact email	chenlong@mail.buct.edu.cn
lab head
Long Chen
contact affiliation	Beijing University of Chemical Technology
contact email	chenlong@mail.buct.edu.cn
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD023315
     1. Label: PRIDE project
     2. Name: Proteomic Analysis of Xinjiang Black Bee's Royal Jelly Using the Osboren Classification Method