PXD023293

PXD023293 is an original dataset announced via ProteomeXchange.

Title	Global Phospho-Proteomic Profiling of Pathway-Level Molecular Alterations in Barrett’s Esophagus
Description	In the present study, we applied deep, quantitative mass-spectrometry to clinical samples (Barrett’s esophagus and matched adjacent normal biopsies) to gain a mechanistic understanding of the molecular pathways associated with disease progression. From our rich LC/MS profiles, we identified a robust proteomic signature that was able to correctly classify independent samples on disease status. Projection of this same signature against EAC tumor profiles was strongly predictive of survival outcomes, while subsequent comparative analysis with published BE transcriptomic profiles provided independent evidence in support of these results. Further, our phosphoproteomic analysis revealed signaling pathways specifically and significantly altered in BE relative to paired controls, providing some mechanistic insights into the cellular dysregulation of key components of specific processes that likely drives disease progression.
HostingRepository	PRIDE
AnnounceDate	2022-08-11
AnnouncementXML	Submission_2022-08-11_05:07:27.740.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Jarrod Moore
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	phosphorylated residue; acetylated residue; iodoacetamide derivatized residue
Instrument	Q Exactive HF-X

Revision	Datetime	Status	ChangeLog Entry
0	2020-12-23 05:17:16	ID requested
⏵ 1	2022-08-11 05:07:28	announced

Moore J, Hekman R, Blum BC, Lawton M, Lehoux S, Stachler M, Pleskow D, Sawhney MS, Cummings RD, Emili A, Qureshi A, Pilot Study Showing Feasibility of Phosphoproteomic Profiling of Pathway-Level Molecular Alterations in Barrett's Esophagus. Genes (Basel), 13(7):(2022) [pubmed]

submitter keyword: Biopsy

Systems Biology

Mass Spectrometry

Disease Signature

Andrew Emili
contact affiliation	Department of Biochemistry at Boston Univeristy School of Medicine
contact email	aemili@bu.edu
lab head
Jarrod Moore
contact affiliation	Boston University School of Medicine
contact email	jmoore5@bu.edu
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2022/08/PXD023293

PRIDE project URI

• PRIDE
  1. PXD023293
     1. Label: PRIDE project
     2. Name: Global Phospho-Proteomic Profiling of Pathway-Level Molecular Alterations in Barrett’s Esophagus