PXD023289

PXD023289 is an original dataset announced via ProteomeXchange.

Title	The GORKY Glycoalkaloid Transporter is Indispensable for Preventing Tomato Bitterness
Description	Fruit taste is determined by sugars, acids and in some species, bitter chemicals. Attraction of seed-dispersing organisms in nature and breeding for consumer preferences requires reduced fruit bitterness. A key metabolic shift during ripening prevents tomato fruit bitterness by eliminating α-tomatine, a renowned defence-associated Solanum alkaloid. Here, we combined fine mapping with information from 150 re-sequenced genomes and genotyping a 650 tomato core collection to identify nine bitter-tasting accessions including the ‘high α-tomatine’ Peruvian landraces reported by Rick and colleagues (1994). These ‘bitter’ accessions contain a deletion in GORKY, a nitrate/peptide family (NPF) transporter mediating α-tomatine subcellular localization during fruit ripening. GORKY exports α-tomatine and its derivatives from the vacuole to the cytosol and this facilitates the conversion of the entire α-tomatine pool to non-bitter forms, rendering the fruit palatable. Hence, GORKY activity was a significant innovation in the process of tomato fruit domestication and breeding. The experiment was carried out to further prove that GORKY is localized to tonoplast in ripe fruit.
HostingRepository	PRIDE
AnnounceDate	2021-01-04
AnnouncementXML	Submission_2021-01-03_23:26:26.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD023289
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Yana Kazachkova
SpeciesList	scientific name: Solanum lycopersicum; NCBI TaxID: 4081;
ModificationList	iodoacetamide derivatized residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2020-12-23 03:52:47	ID requested
⏵ 1	2021-01-03 23:26:27	announced

Dataset with its publication pending

submitter keyword: tomato, fruit, SGA, bitter, transporter, NPF

Asaph Aharoni
contact affiliation	Weizmann Institute of Science, Department of Plant and Environmental Sciences
contact email	asaph.aharoni@weizmann.ac.il
lab head
Yana Kazachkova
contact affiliation	Weizmann Institute of Science
contact email	yana.kazachkova@weizmann.ac.il
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD023289
     1. Label: PRIDE project
     2. Name: The GORKY Glycoalkaloid Transporter is Indispensable for Preventing Tomato Bitterness