PXD023283 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Secretome analysis of Arabidopsis thaliana and Trichoderma atroviride reveals a diverse and dynamic repertoire of extracellular enzymes that may play a role during symbiotic interaction |
Description | The establishment of plant-mutualistic fungi interaction requires a bidirectional molecular crosstalk. The study of their secretomes help to understand a beneficial relationship. Here, a gel-free shotgun proteomic approach was used to identify secreted proteins from semi hydroponic culture systems with Arabidopsis (Arabidopsis thaliana) and T. atroviride alone or in co-culture over a time course (24, 48 and 96h). A total of 126 proteins of Arabidopsis and 1027 proteins of T. atroviride were identified, 118 and 780 of which were differentially modulated in response to their interaction, respectively. Bioinformatic analysis revealed that the secretomes of both organisms were enriched in proteins with a predicted enzymatic function. Among these, increased abundance of putative glycosidases, aspartic endopeptidases and dehydrogenases, together with decreased abundance of amidases, protein-serine/threonine kinases and hydro-lyases was observed in T. atroviride secretome in response to the plant, whereas increased abundances of peroxidases, cysteine endopeptidases and enzymes related to catabolism of secondary metabolites, together with decreased abundance of pathogenesis-related proteins and protease inhibitors was observed in the plant secretome in response to the fungus. Finally, the plant alanine-glyoxylate transaminase GGAT1, increased in abundance, is required for the systemic disease resistance against the phytopathogen Botrytis cinerea but not for plant growth stimulation by T. atroviride |
HostingRepository | PRIDE |
AnnounceDate | 2021-09-09 |
AnnouncementXML | Submission_2021-09-09_15:37:59.420.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Tamar Ziv |
SpeciesList | scientific name: Arabidopsis thaliana (Mouse-ear cress); NCBI TaxID: 3702; scientific name: Trichoderma atroviride; NCBI TaxID: 63577; |
ModificationList | monohydroxylated residue; iodoacetamide derivatized residue |
Instrument | Q Exactive |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2020-12-23 01:52:12 | ID requested | |
⏵ 1 | 2021-09-09 15:38:00 | announced | |
Publication List
Gonz, á, lez-L, ó, pez MDC, Jij, ó, n-Moreno S, Dautt-Castro M, Ovando-V, á, zquez C, Ziv T, Horwitz BA, Casas-Flores S, . Int J Mol Sci, 22(13):(2021) [pubmed] |
Keyword List
submitter keyword: Secretome, Trichoderma, symbiotic interaction |
Contact List
Casas-Flores, S |
contact affiliation | IPICYT, Department of Molecular Biology. Camino a la Presa San José No. 2055. Col. Lomas 4ª. Section, San Luis Potosí, S.L.P., México. C.P. 78216. |
contact email | scasas@ipicyt.edu.mx |
lab head | |
Tamar Ziv |
contact affiliation | Technion |
contact email | tamarz@technion.ac.il |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2021/09/PXD023283 |
PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD023283
- Label: PRIDE project
- Name: Secretome analysis of Arabidopsis thaliana and Trichoderma atroviride reveals a diverse and dynamic repertoire of extracellular enzymes that may play a role during symbiotic interaction