PXD023280

PXD023280 is an original dataset announced via ProteomeXchange.

Title	Sickle cell trait modulates the proteome and phosphoproteome of Plasmodium falciparum-infected erythrocyte
Description	The high prevalence of sickle cell disease in some human populations likely results from the protection afforded against severe Plasmodium falciparum malaria and death by heterozygous carriage of HbS. P. falciparum remodels the erythrocyte membrane and skeleton, displaying parasite proteins at the erythrocyte surface that interact with key human proteins in the Ankyrin R and 4.1R complexes. Oxidative stress generated by HbS, as well as by parasite invasion, disrupts the kinase/phosphatase balance, potentially interfering with the molecular interactions between human and parasite proteins. HbS is known to be associated with abnormal membrane display of parasite antigens. Studying the proteome and the phosphoproteome of red cell membrane extracts from P. falciparum infected and non-infected erythrocytes, we show here that HbS heterozygous carriage, combined with infection, modulates the phosphorylation of erythrocyte membrane transporters and skeletal proteins as well as of parasite proteins. Our results highlight modifications of Ser- /Thr- and/or Tyr- phosphorylation in key human proteins, such as ankyrin, β-adducin, β-spectrin and Band 3, and key parasite proteins, such as RESA or MESA. Altered phosphorylation patterns could disturb the interactions within membrane protein complexes, affect nutrient uptake and the infected erythrocyte cytoadherence phenomenon, thus lessening the severity of malaria symptoms.
HostingRepository	PRIDE
AnnounceDate	2021-04-19
AnnouncementXML	Submission_2021-04-19_04:43:04.035.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Cerina Chhuon
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606; scientific name: Plasmodium falciparum (isolate 3D7); NCBI TaxID: 36329;
ModificationList	phosphorylated residue; monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Q Exactive Plus

Revision	Datetime	Status	ChangeLog Entry
0	2020-12-22 10:02:10	ID requested
1	2021-04-19 04:20:37	announced
⏵ 2	2021-04-19 04:43:04	announced	2021-04-19: Updated project metadata.

10.3389/FCIMB.2021.637604;

Chauvet M, Chhuon C, Lipecka J, Dechavanne S, Dechavanne C, Lohezic M, Ortalli M, Pineau D, Ribeil JA, Manceau S, Le Van Kim C, Luty AJF, Migot-Nabias F, Azouzi S, Guerrera IC, Merckx A, -Infected Erythrocytes. Front Cell Infect Microbiol, 11():637604(2021) [pubmed]

submitter keyword: Plasmodium falciparum, membrane phosphorylation, erythrocyte, hemoglobin S, proteomics.

Anaïs Merckx
contact affiliation	Université de Paris, IRD, UMR 261 MERIT, F-75006 Paris, France
contact email	anais.merckx@parisdescartes.fr
lab head
Cerina Chhuon
contact affiliation	Proteomics Platform Necker, PPN-3P5, Structure Fédérative de Recherche SFR Necker, Université Paris Descartes, Paris, France
contact email	cerina.chhuon@inserm.fr
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2021/04/PXD023280

PRIDE project URI

• PRIDE
  1. PXD023280
     1. Label: PRIDE project
     2. Name: Sickle cell trait modulates the proteome and phosphoproteome of Plasmodium falciparum-infected erythrocyte