PXD023273

PXD023273 is an original dataset announced via ProteomeXchange.

Title	Proteomics Complementation of the Rat Uterotrophic Assay for Estrogenic Endocrine Disruptors: A Roadmap of Advancing High Resolution Mass Spectrometry-based Shotgun Survey to Targeted Biomarker Quantifications Authors: Laszlo Prokai *, Fatima Rahlouni, Khadiza Zaman,Vien Nguyen, and Katalin Prokai-Tatrai
Description	The widely used rat uterotrophic assay to assess known and potential estrogenic compounds only considers uterine weight gain as endpoint measurement. To complement this method with an advanced technology that reveals molecular targets, we analyzed changes in protein expression using label-free quantitative proteomics by liquid chromatography–mass spectrometry on a high resolution (Orbitrap) instrument. Our samples were uterine protein extracts of ovariectomized rats after daily 17β-estradiol exposure for five days in comparison with those of vehicle-treated control animals. The study revealed that __ uterine proteins significantly regulated by estrogen treatment, and crucial findings were verified using multiple reaction monitoring-based targeted proteomics. When mapped by pathway analyses, estrogen-regulated proteins represented cell death and survival, cellular movement and protein synthesis as top molecular and cellular functions, and networks were found with the presence of nuclear estrogen receptor(s) as a prominent molecular node confirmed the relevance of our findings to hormone-associated events.
HostingRepository	PRIDE
AnnounceDate	2021-04-23
AnnouncementXML	Submission_2021-04-22_21:54:30.416.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD023273
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Laszlo Prokai
SpeciesList	scientific name: Rattus norvegicus (Rat); NCBI TaxID: 10116;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue; deamidated residue
Instrument	LTQ Orbitrap Velos

Revision	Datetime	Status	ChangeLog Entry
0	2020-12-22 04:26:53	ID requested
⏵ 1	2021-04-22 21:54:31	announced

Prokai L, Rahlouni F, Zaman K, Nguyen V, Prokai-Tatrai K, Proteomics Complementation of the Rat Uterotrophic Assay for Estrogenic Endocrine Disruptors: A Roadmap of Advancing High Resolution Mass Spectrometry-Based Shotgun Survey to Targeted Biomarker Quantifications. Int J Mol Sci, 22(4):(2021) [pubmed]

submitter keyword: strogen-regulated proteins

rat

uterus

liquid chromatography–mass spectrometry quantitative label-free proteomics

targeted pathway analysis

protein networks

endocrine disruption

Laszlo Prokai, Ph.D, D.Sc
contact affiliation	Professor and Robert A. Welch Chair in Biochemistry Department of Pharmacology and Neuroscience University of North Texas Health Science Center at FortWorth, TX, USA
contact email	Laszlo.Prokai@unthsc.edu
lab head
Laszlo Prokai
contact affiliation	Department of Pharmacology & Neuroscience UNT Health Science Center Fort Worth, TX, USA
contact email	advancedms@unthsc.edu
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2021/04/PXD023273

PRIDE project URI

• PRIDE
  1. PXD023273
     1. Label: PRIDE project
     2. Name: Proteomics Complementation of the Rat Uterotrophic Assay for Estrogenic Endocrine Disruptors: A Roadmap of Advancing High Resolution Mass Spectrometry-based Shotgun Survey to Targeted Biomarker Quantifications Authors: Laszlo Prokai *, Fatima Rahlouni, Khadiza Zaman,Vien Nguyen, and Katalin Prokai-Tatrai