PXD023270

PXD023270 is an original dataset announced via ProteomeXchange.

Title	Crotonylation versus acetylation in PaACL-silenced corollas in petunia
Description	Protein acetylation and crotonylation are important post-translational modifications (PTMs) of lysine. In animal cells, histone lysines are crotonylated or acetylated depends on the relative intracellular concentrations of crotonyl-CoA and acetyl-CoA, and adding crotonate to cell cultures, or reducing the intracellular levels of acetyl-CoA, leads to an increase in histone crotonylation through the direct production of crotonyl-CoA. However, in plant the relationship of acetylation and crotonylation and the effect of the concentration of acetyl-CoA on protein crotonylation were not well known. Our previous study showed that PhACL silencing changed the content of acetyl CoA in petunia corollas. In this study, we performed a global crotonylation proteome analysis of petunia (Petunia hybrida) and found that protein crotonylation have close relationship with protein acetylation and the protein with more crotonylation sites often had more acetylation sites. Crotonylated proteins and acetylated proteins enriched in many common KEGG pathway. However, PhACL silencing resulted in different KEGG pathway enrichment of proteins with different level of crotonylated sites and acetylated sites. Cytoplasm non-histone lysine crotonylation may not depend on the concentrations of acetyl-CoA in petunia corollas. There was a positive correlation between crotonylome and acetylome expression levels in corollas of PhACL-silenced plants and control.
HostingRepository	PRIDE
AnnounceDate	2023-11-14
AnnouncementXML	Submission_2023-11-14_06:37:26.337.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Yixun Yu
SpeciesList	scientific name: Petunia hybrid cultivar; NCBI TaxID: 300558;
ModificationList	acetylated residue
Instrument	Q Exactive Plus

Revision	Datetime	Status	ChangeLog Entry
0	2020-12-22 04:24:53	ID requested
1	2022-10-12 08:51:01	announced
⏵ 2	2023-11-14 06:37:31	announced	2023-11-14: Updated project metadata.

Yang W, Li X, Jiang G, Long Y, Li H, Yu S, Zhao H, Liu J, Crotonylation versus acetylation in petunia corollas with reduced acetyl-CoA due to PaACL silencing. Physiol Plant, 174(5):e13794(2022) [pubmed]

submitter keyword: Crotonylation, PaACL, Petunia

Crotonylome, Gene silencing, Acetylation

Yixun Yu
contact affiliation	South China Agricultural University
contact email	yuyixun@scau.edu.cn
lab head
Yixun Yu
contact affiliation	Guangdong Key Laboratory for Innovative Development and Utilization of Forest Plant Germplasm, College of Forestry and Landscape Architecture, South China Agricultural University, Guangzhou 510642, China
contact email	yuyixun@scau.edu.cn
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD023270
     1. Label: PRIDE project
     2. Name: Crotonylation versus acetylation in PaACL-silenced corollas in petunia