PXD023261

PXD023261 is an original dataset announced via ProteomeXchange.

Title	Hepatic proteomic analysis of selenoprotein T knockout mice by TMT: Implications for the role of selenoprotein T in glucose and lipid metabolism
Description	Selenoprotein T (SELENOT, SelT), a thioredoxin-like enzyme, exerts an essential oxidoreductase activity in the endoplasmic reticulum. However, its precise function remains unknown. To gain more understanding of SELENOT function, a conventional SELENOT knockout (KO) mouse model was constructed for the first time by CRISPR/Cas9 technique. TMT proteomics analysis was conducted to explore the differentially expressed proteins (DEPs) in the liver, revealing 60 up-regulated and 94 down-regulated DEPs in KO mice. The results of proteomics were validated by western blot of 3 selected DEPs (Gys2, DIO1, Gsta2). Furthermore, the bioinformatics analysis showed that SELENOT KO-induced DEPs were mainly related to lipid metabolism, cancer and PPAR signaling pathway. Overall, these findings provide a holistic perspective into SELENOT KO-induced DEPs in the liver and novel insights into the role of SELENOT in glucose and lipid metabolism, and thus enhance our understanding of SELENOT function.
HostingRepository	PRIDE
AnnounceDate	2021-09-09
AnnouncementXML	Submission_2021-09-09_15:26:18.315.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Ke Li
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationList	No PTMs are included in the dataset
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2020-12-21 11:49:41	ID requested
⏵ 1	2021-09-09 15:26:19	announced

Li K, Feng T, Liu L, Liu H, Huang K, Zhou J, Hepatic Proteomic Analysis of Selenoprotein T Knockout Mice by TMT: Implications for the Role of Selenoprotein T in Glucose and Lipid Metabolism. Int J Mol Sci, 22(16):(2021) [pubmed]

submitter keyword: Selenoprotein T

Knockout

Proteomics

TMT

Glucose and lipid metabolism

Jun Zhou
contact affiliation	Hubei Key Laboratory of Bioinorganic Chemistry & Materia Medica, School of Chemistry and Chemical Engineering, Huazhong University of Science and Technology, China
contact email	hustzhj@hust.edu.cn
lab head
Ke Li
contact affiliation	Huazhong University of Science and Technology
contact email	1129715730@qq.com
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD023261
     1. Label: PRIDE project
     2. Name: Hepatic proteomic analysis of selenoprotein T knockout mice by TMT: Implications for the role of selenoprotein T in glucose and lipid metabolism