PXD023261 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Hepatic proteomic analysis of selenoprotein T knockout mice by TMT: Implications for the role of selenoprotein T in glucose and lipid metabolism |
Description | Selenoprotein T (SELENOT, SelT), a thioredoxin-like enzyme, exerts an essential oxidoreductase activity in the endoplasmic reticulum. However, its precise function remains unknown. To gain more understanding of SELENOT function, a conventional SELENOT knockout (KO) mouse model was constructed for the first time by CRISPR/Cas9 technique. TMT proteomics analysis was conducted to explore the differentially expressed proteins (DEPs) in the liver, revealing 60 up-regulated and 94 down-regulated DEPs in KO mice. The results of proteomics were validated by western blot of 3 selected DEPs (Gys2, DIO1, Gsta2). Furthermore, the bioinformatics analysis showed that SELENOT KO-induced DEPs were mainly related to lipid metabolism, cancer and PPAR signaling pathway. Overall, these findings provide a holistic perspective into SELENOT KO-induced DEPs in the liver and novel insights into the role of SELENOT in glucose and lipid metabolism, and thus enhance our understanding of SELENOT function. |
HostingRepository | PRIDE |
AnnounceDate | 2021-09-09 |
AnnouncementXML | Submission_2021-09-09_15:26:18.315.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Ke Li |
SpeciesList | scientific name: Mus musculus (Mouse); NCBI TaxID: 10090; |
ModificationList | No PTMs are included in the dataset |
Instrument | Q Exactive HF |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2020-12-21 11:49:41 | ID requested | |
⏵ 1 | 2021-09-09 15:26:19 | announced | |
Publication List
Li K, Feng T, Liu L, Liu H, Huang K, Zhou J, Hepatic Proteomic Analysis of Selenoprotein T Knockout Mice by TMT: Implications for the Role of Selenoprotein T in Glucose and Lipid Metabolism. Int J Mol Sci, 22(16):(2021) [pubmed] |
Keyword List
submitter keyword: Selenoprotein T |
Knockout |
Proteomics |
TMT |
Glucose and lipid metabolism |
Contact List
Jun Zhou |
contact affiliation | Hubei Key Laboratory of Bioinorganic Chemistry & Materia Medica, School of Chemistry and Chemical Engineering, Huazhong University of Science and Technology, China |
contact email | hustzhj@hust.edu.cn |
lab head | |
Ke Li |
contact affiliation | Huazhong University of Science and Technology |
contact email | 1129715730@qq.com |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD023261
- Label: PRIDE project
- Name: Hepatic proteomic analysis of selenoprotein T knockout mice by TMT: Implications for the role of selenoprotein T in glucose and lipid metabolism