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PXD023261

PXD023261 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleHepatic proteomic analysis of selenoprotein T knockout mice by TMT: Implications for the role of selenoprotein T in glucose and lipid metabolism
DescriptionSelenoprotein T (SELENOT, SelT), a thioredoxin-like enzyme, exerts an essential oxidoreductase activity in the endoplasmic reticulum. However, its precise function remains unknown. To gain more understanding of SELENOT function, a conventional SELENOT knockout (KO) mouse model was constructed for the first time by CRISPR/Cas9 technique. TMT proteomics analysis was conducted to explore the differentially expressed proteins (DEPs) in the liver, revealing 60 up-regulated and 94 down-regulated DEPs in KO mice. The results of proteomics were validated by western blot of 3 selected DEPs (Gys2, DIO1, Gsta2). Furthermore, the bioinformatics analysis showed that SELENOT KO-induced DEPs were mainly related to lipid metabolism, cancer and PPAR signaling pathway. Overall, these findings provide a holistic perspective into SELENOT KO-induced DEPs in the liver and novel insights into the role of SELENOT in glucose and lipid metabolism, and thus enhance our understanding of SELENOT function.
HostingRepositoryPRIDE
AnnounceDate2021-09-09
AnnouncementXMLSubmission_2021-09-09_15:26:18.315.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterKe Li
SpeciesList scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationListNo PTMs are included in the dataset
InstrumentQ Exactive HF
Dataset History
RevisionDatetimeStatusChangeLog Entry
02020-12-21 11:49:41ID requested
12021-09-09 15:26:19announced
Publication List
Li K, Feng T, Liu L, Liu H, Huang K, Zhou J, Hepatic Proteomic Analysis of Selenoprotein T Knockout Mice by TMT: Implications for the Role of Selenoprotein T in Glucose and Lipid Metabolism. Int J Mol Sci, 22(16):(2021) [pubmed]
Keyword List
submitter keyword: Selenoprotein T
Knockout
Proteomics
TMT
Glucose and lipid metabolism
Contact List
Jun Zhou
contact affiliationHubei Key Laboratory of Bioinorganic Chemistry & Materia Medica, School of Chemistry and Chemical Engineering, Huazhong University of Science and Technology, China
contact emailhustzhj@hust.edu.cn
lab head
Ke Li
contact affiliationHuazhong University of Science and Technology
contact email1129715730@qq.com
dataset submitter
Full Dataset Link List
Dataset FTP location
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PRIDE project URI
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