PXD023256

PXD023256 is an original dataset announced via ProteomeXchange.

Title	Blocking ERK activity has a selective but critical impact on activating T cells
Description	The mitogen activated kinases ERK1/2 are activated by antigen receptor engagement and control T cell differentiation. We have used mass spectrometry to explore how ERK1/2 control antigen receptor driven cell growth and proteome restructuring in CD8 T cells. Quantitative analysis of >8000 proteins provides new understanding of the highly selective role of ERK1/2 in controlling T cell protein systems. The data reveal that ERK signalling is not a dominant regulator of the metabolic and biosynthetic programs that drive T cell growth. Rather, the dominant function of ERK1/2 is to control the repertoire of transcription factors, cytokines and cytokine receptors expressed by activated T cells. This study provides a comprehensive map of how T cell phenotypes are selectively shaped by ERK1/2 and reveals that ERK1/2 controls the transcriptional reprogramming of activated T cells that is pivotal for T cell differentiation and acquisition of effector function.
HostingRepository	PRIDE
AnnounceDate	2020-12-23
AnnouncementXML	Submission_2020-12-23_02:13:24.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Andrew Howden
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationList	No PTMs are included in the dataset
Instrument	LTQ Orbitrap Velos

Revision	Datetime	Status	ChangeLog Entry
0	2020-12-21 11:46:15	ID requested
⏵ 1	2020-12-23 02:13:24	announced

Dataset with its publication pending

submitter keyword: MAPK, ERK1/2, T cells, immunology

Professor Doree Cantrell
contact affiliation	Cell Signalling and Immunology, School of Life Sciences, University of Dundee, Dundee, UK
contact email	d.a.cantrell@dundee.ac.uk
lab head
Andrew Howden
contact affiliation	University of Dundee
contact email	a.howden@dundee.ac.uk
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2020/12/PXD023256

PRIDE project URI

• PRIDE
  1. PXD023256
     1. Label: PRIDE project
     2. Name: Blocking ERK activity has a selective but critical impact on activating T cells