PXD023254

PXD023254 is an original dataset announced via ProteomeXchange.

Dataset Summary

Title	Identification of novel potential interaction partners of UDP-galactose (SLC35A2), UDP-N-acetylglucosamine (SLC35A3) and an orphan (SLC35A4) nucleotide sugar transporters
Description	Nucleotide sugar transporters (NSTs) are ER and Golgi-resident members of the solute carrier 35 (SLC35) family which supply substrates for glycosylation by exchanging lumenal nucleotide monophosphates for cytosolic nucleotide sugars. Defective NSTs have been associated with congenital disorders of glycosylation (CDG), however, molecular basis of many types of CDG remains poorly characterized. To better understand CDG biology, we identified potential interaction partners of UDP-galactose transporter (SLC35A2), UDP-N-acetylglucosamine transporter (SLC35A3) and an orphan nucleotide sugar transporter SLC35A4. For this purpose, each of the SLC35A2-A4 proteins was used as a bait in four independent pull-down experiments and the identity of the immunoprecipitated material was discovered using MS techniques. The consensus findings for each of the NSTs tested were listed as potential interaction partners and should prove useful as a starting point for deciphering the NST interaction network.
HostingRepository	MassIVE
AnnounceDate	2022-01-04
AnnouncementXML	Submission_2022-01-04_04:33:44.034.xml
DigitalObjectIdentifier
ReviewLevel	Non peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Maciej Wiktor
SpeciesList	scientific name: Homo sapiens; common name: human; NCBI TaxID: 9606;
ModificationList	Acetyl; L-methionine sulfoxide; methionine oxidation with neutral loss of 64 Da; O-phospho-L-serine; O-phospho-L-threonine; O4'-phospho-L-tyrosine; phosphorylated residue with neutral loss of phosphate
Instrument	LTQ Orbitrap Velos

Dataset History

Revision	Datetime	Status	ChangeLog Entry
0	2020-12-21 07:58:05	ID requested
⏵ 1	2022-01-04 04:33:44	announced

Publication List

no publication

no publication

Keyword List

submitter keyword: nucleotide sugar transporters, UDP-galactose transporter, UDP-N-acetylglucosamine transporter, SLC35A subfamily of proteins, glycosylation, endoplasmic reticulum, Golgi apparatus

submitter keyword: nucleotide sugar transporters, UDP-galactose transporter, UDP-N-acetylglucosamine transporter, SLC35A subfamily of proteins, glycosylation, endoplasmic reticulum, Golgi apparatus

Contact List

Maciej Wiktor
contact affiliation	University of Wroclaw
contact email	maciej.wiktor@uwr.edu.pl
lab head
Maciej Wiktor
contact affiliation	University of Wroclaw
contact email	maciej.wiktor@uwr.edu.pl
dataset submitter

Full Dataset Link List

MassIVE dataset URI
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MassIVE dataset URI

Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://massive.ucsd.edu/MSV000086622/