PXD023238 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | TENT5 cytoplasmic non-canonical poly(A) polymerases regulate the innate immune response in animals |
Description | Innate immunity, the first line of host defense against pathogens, is tightly regulated both transcriptionally and post-transcriptionally. Here, through global transcriptome and proteome analyses in Caenorhabditis elegans, we uncover a modulation of the expression of secreted innate immunity effector proteins by TENT5, one of a recently described family of cytoplasmic poly(A) polymerases. Direct RNA sequencing revealed that TENT-5 polyadenylates mRNAs with signal peptide-encoding sequences, that are translated at the endoplasmic reticulum. Loss of tent-5 function makes worms more susceptible to bacterial infection. Importantly, we demonstrate that the function of TENT-5 in innate immunity is evolutionarily conserved, as its orthologs, TENT5A and TENT5C are induced during macrophage activation and polyadenylate mRNAs, some of which are of genes orthologous to C. elegans TENT-5 targets. In summary, our study reveals cytoplasmic polyadenylation to be a previously unknown component of the post-transcriptional regulation of innate immunity in animals. |
HostingRepository | PRIDE |
AnnounceDate | 2023-11-14 |
AnnouncementXML | Submission_2023-11-14_08:51:52.412.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Dominik Cysewski |
SpeciesList | scientific name: Caenorhabditis elegans; NCBI TaxID: 6239; |
ModificationList | No PTMs are included in the dataset |
Instrument | LTQ Orbitrap Velos |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2020-12-21 02:35:31 | ID requested | |
1 | 2022-05-31 02:39:52 | announced | |
⏵ 2 | 2023-11-14 08:51:53 | announced | 2023-11-14: Updated project metadata. |
Publication List
Dataset with its publication pending |
Keyword List
submitter keyword: innate immunity, cytoplasmic polyadenylation, non-canonical polyadenylation, TENT5, direct RNA sequencing,Terminal nucleotidyltransferase, C. elegans innate immunity |
Contact List
Andrzej Dziembowski |
contact affiliation | Laboratory of RNA Biology International Institute of Molecular and Cell Biology in Warsaw |
contact email | adziembowski@iimcb.gov.pl |
lab head | |
Dominik Cysewski |
contact affiliation | Medical University of Bialystok |
contact email | dominikcysewski@gmail.com |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2022/05/PXD023238 |
PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD023238
- Label: PRIDE project
- Name: TENT5 cytoplasmic non-canonical poly(A) polymerases regulate the innate immune response in animals