PXD023234

PXD023234 is an original dataset announced via ProteomeXchange.

Title	Linking post-translational modifications and protein turnover by site-resolved protein turnover (SPOT) profiling
Description	Proteome-wide measurements of protein turnover have largely ignored the impact of post-translational modifications (PTMs). To address this gap, we employ stable isotope labelling and mass spectrometry to measure the turnover of >120,000 peptidoforms including >33,000 phosphorylated, acetylated, and ubiquitinated peptides for >9,000 native proteins. This site-resolved protein turnover (SPOT) profiling discloses global and site-specific differences in turnover associated with the presence or absence of PTMs. While causal relationships may not always be immediately apparent, we hypothesize that PTMs with diverging turnover may distinguish states of differential protein stability, structure, localization, enzymatic activity, or protein-protein interactions. We exemplify how the turnover data may facilitate insights into unknown functions of PTMs and provide a web-tool for the scientific community that allows interrogation and visualisation of all turnover data. Since the methodology is applicable to many cell types and modifications, it has substantial potential to prioritize PTMs for functional investigation in the future.
HostingRepository	PanoramaPublic
AnnounceDate	2022-03-14
AnnouncementXML	Submission_2022-03-14_20:53:09.155.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	supported by repository but incomplete data and/or metadata
PrimarySubmitter	Julia Mergner
SpeciesList	scientific name: Homo sapiens; NCBI TaxID: 9606;
ModificationList	GG; Acetyl; Phospho; Label:13C(6)15N(2)
Instrument	Q Exactive HF-X; Orbitrap Fusion Lumos

Revision	Datetime	Status	ChangeLog Entry
0	2020-12-18 16:46:05	ID requested
1	2021-11-10 14:24:44	announced
⏵ 2	2022-03-14 20:53:10	announced	: Added publication

Zecha J, Gabriel W, Spallek R, Chang YC, Mergner J, Wilhelm M, Bassermann F, Kuster B, Linking post-translational modifications and protein turnover by site-resolved protein turnover profiling. Nat Commun, 13(1):165(2022) [pubmed]

submitter keyword: Phospho, Ubiquitin, PTMs, proteasome, FBXW7, TKT

Bernhard Kuster
contact affiliation	Chair of Proteomics, and Bioanalytics, Technical University of Munich, Freising, Germany
contact email	kuster@tum.de
lab head
Julia Mergner
contact affiliation	Chair of Proteomics and Bioanalytics, Technical University of Munich, Freising, Germany
contact email	julia.mergner@tum.de
dataset submitter

Panorama Public dataset URI