PXD023233 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Bacteria in biogas digestates for reduced climate forcing |
Description | Mitigation of N2O-emissions from soils is needed to reduce climate forcing by food production. Inoculating soils with N2O-reducing bacteria would be effective, but costly and impractical as a standalone operation. Here we demonstrate that digestates obtained after biogas production may provide a low-cost and widely applicable solution. Firstly, we show that indigenous N2O-reducing bacteria in digestates grow to high levels during anaerobic enrichment under N2O. Gas kinetics and meta-omic analysis show that the N2O respiring organisms, recovered as metagenome-assembled genomes (MAGs) grow by harvesting fermentation intermediates of the methanogenic consortium. Three digestate-derived denitrifying bacteria were obtained through isolation, one of which matched the recovered MAG of a dominant Dechloromonas-affiliated N2O reducer. While the identified N2O-reducers encoded genes required for a full denitrification pathway and could thus both produce and sequester N2O, their regulatory traits predicted that they act as N2O-sinks in the current system. Secondly, moving towards practical application, we show that these isolates grow by aerobic respiration in digestates, and that fertilization with these enriched digestates reduces N2O emissions. This shows that the ongoing implementation of biogas production in agriculture opens a new avenue for cheap and effective reduction of N2O emissions from food production. |
HostingRepository | PRIDE |
AnnounceDate | 2022-02-16 |
AnnouncementXML | Submission_2022-02-16_12:25:46.202.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Live Hagen |
SpeciesList | scientific name: unclassified Azonexus; NCBI TaxID: 2649329; |
ModificationList | acetylated residue; monohydroxylated residue |
Instrument | Q Exactive HF |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2020-12-18 09:14:46 | ID requested | |
⏵ 1 | 2022-02-16 12:25:47 | announced | |
Publication List
Jonassen KR, Hagen LH, Vick SHW, Arntzen M Ø, Eijsink VGH, Frosteg, å, rd Å, Lycus P, Molstad L, Pope PB, Bakken LR, Nitrous oxide respiring bacteria in biogas digestates for reduced agricultural emissions. ISME J, 16(2):580-590(2022) [pubmed] |
Keyword List
submitter keyword: Anaerobic digestion, isolate, Dechloromonas sp, Azonexus sp., denitrification, N2O-reduction, LC-MSMS. |
Contact List
Lars Bakken |
contact affiliation | Faculty of Biotechnology, Chemistry and Food Science, NMBU - Norwegian University of Life Sciences, Norway |
contact email | lars.bakken@nmbu.no |
lab head | |
Live Hagen |
contact affiliation | Faculty of Biotechnology, Chemistry and Food Science, Norwegian University of Life Sciences, Norway |
contact email | live.hagen@nmbu.no |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD023233
- Label: PRIDE project
- Name: Bacteria in biogas digestates for reduced climate forcing