PXD023223

PXD023223 is an original dataset announced via ProteomeXchange.

Title	The label-free quantitative secretome of MCF-7 cells treated with sumaCTX
Description	This study aimed to investigate the alteration of intracellular proteome of MCF-7 cells following membrane permeabilization by sumaCTX at increasing concentration, using a label-free quantitative (LFQ) approach. The secretome represents the leakage of intracellular proteins in MCF- 7 cells were subjected to trypsin digestion and LC-MS/MS. Based on the LFQ secretome, there was a total of 105 distinct abundance proteins (DAPs) identified in different experimental groups. Further bioinformatics analyses revealed that most of these DAPs were confined to cytoplasmic, nucleus, and membrane. The DEPs involved in metabolic pathways, membrane integrity, inflammatory responses, and necroptosis. There were relatively higher detectable levels of DAPs in the secretome treated with a higher concentration of sumaCTX, suggesting prominent membrane permeabilization. The necroptosis was possibly a stress response in MCF-7 upon exposure to the high concentration of sumaCTX.
HostingRepository	PRIDE
AnnounceDate	2021-06-29
AnnouncementXML	Submission_2021-06-29_08:55:42.178.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Michelle Yap
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	No PTMs are included in the dataset
Instrument	6550 iFunnel Q-TOF LC/MS

Revision	Datetime	Status	ChangeLog Entry
0	2020-12-18 05:25:04	ID requested
⏵ 1	2021-06-29 08:55:42	announced

Hiu JJ, Yap MKK, The effects of Naja sumatrana venom cytotoxin, sumaCTX on alteration of the secretome in MCF-7 breast cancer cells following membrane permeabilization. Int J Biol Macromol, 184():776-786(2021) [pubmed]

submitter keyword: LC-MS/MS, LFQ, sumaCTX, necroptosis

Michelle Yap
contact affiliation	School of Science, Monash University Malaysia
contact email	yap.michelle@monash.edu
lab head
Michelle Yap
contact affiliation	Monash University
contact email	yap.michelle@monash.edu
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2021/06/PXD023223

PRIDE project URI

• PRIDE
  1. PXD023223
     1. Label: PRIDE project
     2. Name: The label-free quantitative secretome of MCF-7 cells treated with sumaCTX