PXD023211

PXD023211 is an original dataset announced via ProteomeXchange.

Title	Multiplexed complexome profiling using tandem mass tags
Description	Complexome profiling is a rapidly spreading, powerful approach to gain insight into the nature of protein complexes. It identifies and quantifies protein complexes separated into multiple fractions of increasing molecular mass using mass spectrometry-based, label-free bottom-up proteomics. Complexome profiling enables a sophisticated and thorough characterization of the composition, molecular mass, assembly and interactions of protein complexes. However, in practice, its application is limited by the large number of samples it generates and the related time and costs of mass spectrometry analyses. Here we implemented tandem mass tags for multiplexing complexome profiling.
HostingRepository	PRIDE
AnnounceDate	2021-08-20
AnnouncementXML	Submission_2021-08-20_02:58:49.119.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Christoph Krisp
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	iodoacetamide derivatized residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2020-12-18 01:48:08	ID requested
1	2021-08-20 01:53:47	announced
2	2021-08-20 02:50:01	announced	2021-08-20: Updated project metadata.
⏵ 3	2021-08-20 02:58:49	announced	2021-08-20: Updated project metadata.

10.1016/J.BBABIO.2021.148448;

Guerrero-Castillo S, Krisp C, K, ü, chler K, Arnold S, Schl, ü, ter H, Gersting SW, Multiplexed complexome profiling using tandem mass tags. Biochim Biophys Acta Bioenerg, 1862(9):148448(2021) [pubmed]

submitter keyword: Complexome profiling

multiplexed complexomics

Tandem mass tags

protein complexes

Sergio Guerrero-Castillo
contact affiliation	University Children‘s Research@Kinder-UKE University Medical Center Hamburg-Eppendorf 20246, Hamburg, Germany
contact email	s.guerrerocastillo@uke.de
lab head
Christoph Krisp
contact affiliation	University Medical Center Hamburg-Eppendorf Institute of Clinical Chemistry and Laboratory Medicine Mass Spectrometric Proteomics
contact email	c.krisp@uke.de
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2021/08/PXD023211

PRIDE project URI

• PRIDE
  1. PXD023211
     1. Label: PRIDE project
     2. Name: Multiplexed complexome profiling using tandem mass tags