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PXD023204

PXD023204 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleZinc-associated proteome of Cryptococcus neoformans
DescriptionFungal diseases are critical burdens on the global healthcare system, infecting over 25% of the world’s population. For the opportunistic fungal pathogen, Cryptococcus neoformans, infection leads to cryptococcosis; a disease enabled by elaboration of sophisticated virulence factors, including polysaccharide capsule, melanin, thermotolerance, and extracellular enzymes in the presence of the host. Conversely, the host protects itself from fungal invasion by regulating and sequestering transition metals (e.g., iron, zinc, copper) important for microbial growth and survival. Building upon knowledge of zinc transport regulation at the transcriptional level, here, we explore the intricate relationship between zinc availability and fungal virulence via mass spectrometry-based quantitative proteomics. We observe distinct protein-level responses to zinc-limited and -excess conditions through a shift of the proteome profile towards stress response and metal acquisition, including the upregulation of the zinc transporter, ZIP1. In addition, we reveal a novel connection among zinc availability, protein folding, capsule production, and virulence through the detection of a Wos2-like ortholog in the secretome under replete conditions. Overall, we provide new biological insight into cellular remodeling at the protein level of C. neoformans under regulated zinc conditions and uncover a novel connection between zinc availability and fungal virulence. These findings support the development of new antifungal strategies focused on the enhancement of nutritional immunity within the host.
HostingRepositoryPRIDE
AnnounceDate2022-02-16
AnnouncementXMLSubmission_2022-02-16_12:29:58.359.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterJennifer Geddes-McAlister
SpeciesList scientific name: Cryptococcus neoformans var. grubii H99; NCBI TaxID: 235443;
ModificationListmonohydroxylated residue; acetylated residue; iodoacetamide derivatized residue
InstrumentOrbitrap Fusion Lumos
Dataset History
RevisionDatetimeStatusChangeLog Entry
02020-12-17 11:09:14ID requested
12022-02-16 12:29:59announced
Publication List
Ball B, Woroszchuk E, Sukumaran A, West H, Afaq A, Carruthers-Lay D, Muselius B, Gee L, Langille M, Pladwig S, Kazi S, Hendriks A, Geddes-McAlister J, Proteome and secretome profiling of zinc availability in Cryptococcus neoformans identifies Wos2 as a subtle influencer of fungal virulence determinants. BMC Microbiol, 21(1):341(2021) [pubmed]
Keyword List
submitter keyword: Cryptococcus neoformans, fungal pathogenesis, zinc limitation, quantitative proteomics, polysaccharide capsule, virulence
Contact List
Dr. Jennifer Geddes-McAlister
contact affiliationDepartment of Molecular and Cellular Biology, University of Guelph, Guelph, Ontario, Canada, N1G 2W1, 519-824-4120 ext. 52129
contact emailjgeddesm@uoguelph.ca
lab head
Jennifer Geddes-McAlister
contact affiliationUniversity of Guelph
contact emailjgeddesm@uoguelph.ca
dataset submitter
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