PXD023196

PXD023196 is an original dataset announced via ProteomeXchange.

Title	Mass spectrometry spatial-omics on a single conductive slide
Description	Mass spectrometry imaging (MSI) can analyze the spatial distribution of hundreds of different molecules directly from tis-sue sections usually placed on conductive glass slides to provide conductivity on the sample surface. Additional experiments are often required for molecular identification using consecutive sections on membrane slides compatible with laser capture microdissection (LMD). In this work, we demonstrate for the first time the use of a single conductive slide for both matrix assisted laser desorption ionization (MALDI)-MSI and direct proteomics. In this workflow, regions of interest can be directly ablated with LMD while preserving protein integrity. These results offer an alternative for MSI based multimodal spatial-omics.
HostingRepository	PRIDE
AnnounceDate	2021-09-10
AnnouncementXML	Submission_2021-09-09_16:41:32.086.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Stephanie Mezger
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090; scientific name: Rattus norvegicus (Rat); NCBI TaxID: 10116;
ModificationList	acetylated residue; monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2020-12-17 03:44:51	ID requested
⏵ 1	2021-09-09 16:41:32	announced

Mezger STP, Mingels AMA, Bekers O, Heeren RMA, Cillero-Pastor B, Mass Spectrometry Spatial-Omics on a Single Conductive Slide. Anal Chem, 93(4):2527-2533(2021) [pubmed]

submitter keyword: MALDI-MSI, LMD, proteomics, conductive slide

Ronald Martinus Alexander Heeren
contact affiliation	Maastricht MultiModal Molecular Imaging (M4I) Institute, Division of Imaging Mass Spectrometry, Maastricht University, Maastricht, The Netherlands
contact email	r.heeren@maastrichtuniversity.nl
lab head
Stephanie Mezger
contact affiliation	Maastricht MultiModal Molecular Imaging (M4I) Institute, Division of Imaging Mass Spectrometry, Maastricht University, The Netherlands
contact email	s.mezger@maastrichtuniversity.nl
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2021/09/PXD023196

PRIDE project URI

• PRIDE
  1. PXD023196
     1. Label: PRIDE project
     2. Name: Mass spectrometry spatial-omics on a single conductive slide