PXD023193

PXD023193 is an original dataset announced via ProteomeXchange.

Title	Genetic manipulation of Hmr leads to altered protein-protein interactions network
Description	In this study we use Affinity Purification coupled with Mass Spectrometry (AP-MS) to test the effect of HMR overexpression (Hmr.over) or mutation (Hmr.dC and Hmr.2) on the interactome of HMR and in relation to the Speciation Core Complex (SCC). All affinity purifications were performed with a FLAG antibody to target either the exogenously expressed transgenes (Hmr.over, Hmr.dC, Hmr.2) or an endogenously FLAG-tagged HMR (Hmr.endo). Hmr.dC mutants carry a truncation in the BESS-domain-containing C-terminus of HMR. Hmr.2 carries 2 point mutations towards its N-terminus.
HostingRepository	PRIDE
AnnounceDate	2021-08-11
AnnouncementXML	Submission_2021-08-10_23:06:35.544.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Andrea Lukacs
SpeciesList	scientific name: Drosophila melanogaster (Fruit fly); NCBI TaxID: 7227;
ModificationList	No PTMs are included in the dataset
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2020-12-17 02:55:27	ID requested
⏵ 1	2021-08-10 23:06:35	announced

Dataset with its publication pending

submitter keyword: : chromatin

speciation

nucleolus

centromere

heterochromatin

chromocenter

Drosophila

development

hybrids

Hmr

BESS

HmrdC

Hmr2

Axel Imhof
contact affiliation	Ludwig-Maximilians-University of Munich Biomedical Center - Molecular Biology Großhaderner Strasse 9 82152 Planegg-Martinsried Germany
contact email	imhof@lmu.de
lab head
Andrea Lukacs
contact affiliation	Biomedical Center of the Ludwig Maximilians University of Munich
contact email	ander.lukacs@yahoo.it
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2021/08/PXD023193

PRIDE project URI

• PRIDE
  1. PXD023193
     1. Label: PRIDE project
     2. Name: Genetic manipulation of Hmr leads to altered protein-protein interactions network