PXD023188

PXD023188 is an original dataset announced via ProteomeXchange.

Title	The protein-protein interaction network of the Drosophila Speciation Core Complex
Description	In this study we described the protein-protein interaction network of the Drosophila Speciation Core Complex by analysing the interactome of its subunit: HMR, LHR, NLP, BOH1 (CG33213), BOH2 (CG4788) and HP1a. For this purpose we performed Affinity Purification coupled with Mass Spectrometry (AP-MS) in D. melanogaster SL2 cells using as bait the two hybrid incompatibility proteins HMR (n = 8) and LHR (n = 4), as well as NLP (n = 3), BOH1(CG33213, n = 4), BOH2 (CG4788, n = 5) and HP1a (n = 4). Each bait was targeted with at least one antibody (rat anti-LHR 12F4, mouse anti-HP1a 2C09, rabbit anti-Nlp, anti-FLAG-M2 for FLAG-CG33213 and FLAG-CG4788), while HMR was targeted with three different antibodies (rat anti-HMR 2C10 and 12F1, anti-FLAG-M2 for FLAG-HMR). Individual replicates and antibodies used are listed in samples_table.
HostingRepository	PRIDE
AnnounceDate	2021-08-11
AnnouncementXML	Submission_2021-08-10_23:17:21.531.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Andrea Lukacs
SpeciesList	scientific name: Drosophila melanogaster (Fruit fly); NCBI TaxID: 7227;
ModificationList	No PTMs are included in the dataset
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2020-12-17 02:40:52	ID requested
⏵ 1	2021-08-10 23:17:21	announced

Dataset with its publication pending

submitter keyword: chromatin

speciation

nucleolus

centromere

heterochromatin

chromocenter

Drosophila

development

hybrids

NLP

NPH

nucleoplasmin

nucleophosmin

HP1a

HMR

LHR

BOH1

BOH2;

Axel Imhof
contact affiliation	Ludwig-Maximilians-University of Munich Biomedical Center - Molecular Biology Großhaderner Strasse 9 82152 Planegg-Martinsried Germany
contact email	imhof@lmu.de
lab head
Andrea Lukacs
contact affiliation	Biomedical Center of the Ludwig Maximilians University of Munich
contact email	ander.lukacs@yahoo.it
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2021/08/PXD023188

PRIDE project URI

• PRIDE
  1. PXD023188
     1. Label: PRIDE project
     2. Name: The protein-protein interaction network of the Drosophila Speciation Core Complex