PXD023186 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | LC-MS/MS of autotrophic culture KS, that performs nitrate reduction coupled to iron(II) oxidation (NDFO) |
Description | Nitrate-reducing iron(II)-oxidizing bacteria are widespread in the environment contribute to nitrate removal and influence the fate of the greenhouse gases nitrous oxide and carbon dioxide. The autotrophic growth of nitrate-reducing iron(II)-oxidizing bacteria is rarely investigated and poorly understood. The most prominent model system for this type of studies is enrichment culture KS, which originates from a freshwater sediment in Bremen, Germany. To gain insights in the metabolism of nitrate reduction coupled to iron(II) oxidation under in the absence of organic carbon and oxygen limited conditions, we performed metagenomic, metatranscriptomic and metaproteomic analyses of culture KS. Raw sequencing data of 16S rRNA amplicon sequencing, shotgun metagenomics (short reads: Illumina; long reads: Oxford Nanopore Technologies), metagenome assembly, raw sequencing data of shotgun metatranscriptomes (2 conditions, triplicates) can be found at SRA in https://www.ncbi.nlm.nih.gov/bioproject/PRJNA682552. This dataset contains proteomics data for 2 conditions (heterotrophic and autotrophic growth conditions) in triplicates. |
HostingRepository | PRIDE |
AnnounceDate | 2021-05-17 |
AnnouncementXML | Submission_2021-05-17_12:18:40.296.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Daniel Straub |
SpeciesList | scientific name: freshwater sediment metagenome; NCBI TaxID: 556182; |
ModificationList | No PTMs are included in the dataset |
Instrument | Q Exactive HF |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2020-12-17 02:40:39 | ID requested | |
⏵ 1 | 2021-05-17 12:18:40 | announced | |
Publication List
Dataset with its publication pending |
Keyword List
submitter keyword: culture KS, iron oxidation, nitrate reduction, NDFO, autotrohpic, heterotrophic |
Contact List
Sara Kleindienst |
contact affiliation | Microbial Ecology, Center for Applied Geoscience, University of Tuebingen, Germany |
contact email | sara.kleindienst@uni-tuebingen.de |
lab head | |
Daniel Straub |
contact affiliation | University of Tübingen |
contact email | daniel.straub@uni-tuebingen.de |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD023186
- Label: PRIDE project
- Name: LC-MS/MS of autotrophic culture KS, that performs nitrate reduction coupled to iron(II) oxidation (NDFO)