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PXD023186

PXD023186 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleLC-MS/MS of autotrophic culture KS, that performs nitrate reduction coupled to iron(II) oxidation (NDFO)
DescriptionNitrate-reducing iron(II)-oxidizing bacteria are widespread in the environment contribute to nitrate removal and influence the fate of the greenhouse gases nitrous oxide and carbon dioxide. The autotrophic growth of nitrate-reducing iron(II)-oxidizing bacteria is rarely investigated and poorly understood. The most prominent model system for this type of studies is enrichment culture KS, which originates from a freshwater sediment in Bremen, Germany. To gain insights in the metabolism of nitrate reduction coupled to iron(II) oxidation under in the absence of organic carbon and oxygen limited conditions, we performed metagenomic, metatranscriptomic and metaproteomic analyses of culture KS. Raw sequencing data of 16S rRNA amplicon sequencing, shotgun metagenomics (short reads: Illumina; long reads: Oxford Nanopore Technologies), metagenome assembly, raw sequencing data of shotgun metatranscriptomes (2 conditions, triplicates) can be found at SRA in https://www.ncbi.nlm.nih.gov/bioproject/PRJNA682552. This dataset contains proteomics data for 2 conditions (heterotrophic and autotrophic growth conditions) in triplicates.
HostingRepositoryPRIDE
AnnounceDate2021-05-17
AnnouncementXMLSubmission_2021-05-17_12:18:40.296.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterDaniel Straub
SpeciesList scientific name: freshwater sediment metagenome; NCBI TaxID: 556182;
ModificationListNo PTMs are included in the dataset
InstrumentQ Exactive HF
Dataset History
RevisionDatetimeStatusChangeLog Entry
02020-12-17 02:40:39ID requested
12021-05-17 12:18:40announced
Publication List
Dataset with its publication pending
Keyword List
submitter keyword: culture KS, iron oxidation, nitrate reduction, NDFO, autotrohpic, heterotrophic
Contact List
Sara Kleindienst
contact affiliationMicrobial Ecology, Center for Applied Geoscience, University of Tuebingen, Germany
contact emailsara.kleindienst@uni-tuebingen.de
lab head
Daniel Straub
contact affiliationUniversity of Tübingen
contact emaildaniel.straub@uni-tuebingen.de
dataset submitter
Full Dataset Link List
Dataset FTP location
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PRIDE project URI
Repository Record List
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