PXD023176 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | The dipeptide prolyl-hydroxyproline promotes cellular homeostasis and lamellipodia-driven motility via active ?1-integrin in adult tendon cells |
Description | Collagen-derived hydroxyproline-containing peptides show a variety of biological properties in cells. However, no comprehensive study has yet explored the functional links between Hyp-containing peptides and cellular behavior. Here, we show that the dipeptide prolyl-hydroxyproline (Pro–Hyp) exhibits the most significant effect of a number of di- and tri-peptides on mouse tendon cells. We have shown that the cellular uptake of Pro-Hyp is a carrier-mediated process. In addition, Pro-Hyp promotes differentiation/maturation of tendon cells with modulation of lineage-specific factors, has profound effects on cellular phenotypes such as cell proliferation with significantly upregulated ERK-phosphorylation and extracellular matrix production with increased type I collagen network organization, and induced significant chemotactic activity in vitro. Proteomics analysis has identified cellular assembly and organizations, and movement as predicted linked-network pathways in response to Pro-Hyp. Mechanistically, cells treated with Pro-Hyp demonstrate increased directional persistence and significantly increased directed motility and migration velocity, which are accompanied by elongated lamellipodial protrusions with increased active β1-integrin-containing focal contacts and reorganizations of thicker peripheral F-actin fibrils. Thus, our findings document the molecular basis of the functional benefits of Pro-Hyp dipeptide in cellular behavior. Such dynamic properties of collagen-derived Pro-Hyp dipeptide could lead the way to its application to translational medicine. |
HostingRepository | PRIDE |
AnnounceDate | 2021-05-27 |
AnnouncementXML | Submission_2021-05-26_23:29:16.070.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Rosalind Jenkins |
SpeciesList | scientific name: Mus musculus (Mouse); NCBI TaxID: 10090; |
ModificationList | methylthiolated residue; monohydroxylated residue; acetylated residue; deamidated residue |
Instrument | TripleTOF 6600 |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2020-12-16 02:48:10 | ID requested | |
⏵ 1 | 2021-05-26 23:29:16 | announced | |
Publication List
Dataset with its publication pending |
Keyword List
submitter keyword: Collagen-derived peptide |
Proline-hydroxyproline |
Adult tendon cells |
Extracellular matrix |
Scleraxis |
Cell motility |
Extracellular signal-regulated kinase |
Contact List
Dr Rosalind Jenkins |
contact affiliation | Centre for Drug Safety Science, Dept Pharmacology and Therapeutics, Institute of Systems, Molecular and Integrative Biology, University of Liverpool |
contact email | R.Jenkins@liv.ac.uk |
lab head | |
Rosalind Jenkins |
contact affiliation | University of Liverpool |
contact email | R.Jenkins@liv.ac.uk |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD023176
- Label: PRIDE project
- Name: The dipeptide prolyl-hydroxyproline promotes cellular homeostasis and lamellipodia-driven motility via active ?1-integrin in adult tendon cells