PXD023171

PXD023171 is an original dataset announced via ProteomeXchange.

Title	Trait tacking for predictive evolution of metabolic pheotypes
Description	Adaptive laboratory evolution is highly effective for improving desired traits through natural selection. However, its applicability is inherently constrained to growth-correlated traits precluding traits of interest that incur a fitness cost, such as metabolite secretion. Here, we introduce the concept of tacking trait enabling natural selection of fitness-costly metabolic traits. The concept is inspired from the tacking maneuver used in sailing for traversing upwind. We use first-principle metabolic models to design an evolution niche wherein the tacking trait and fitness become correlated. Adaptive evolution in this niche, when followed by the reversal to the original niche, manifests in the improvement of the desired trait due to biochemical coupling between the tacking and the desired trait. We experimentally demonstrate this strategy, termed EvolveX, by evolving wine yeasts for increased aroma production. Our results pave the way for precision laboratory evolution for biotechnological and ecological applications.
HostingRepository	PRIDE
AnnounceDate	2022-07-12
AnnouncementXML	Submission_2022-07-12_05:07:22.366.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Frank Stein
SpeciesList	scientific name: Saccharomyces cerevisiae (Baker's yeast); NCBI TaxID: 4932;
ModificationList	TMT6plex-126 reporter+balance reagent acylated residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2020-12-16 01:31:57	ID requested
⏵ 1	2022-07-12 05:07:22	announced

Dataset with its publication pending

submitter keyword: ALE S. cerevisiae wine aroma

Kiran Raosaheb Patil
contact affiliation	EMBL Heidelberg
contact email	patil@embl.de
lab head
Frank Stein
contact affiliation	EMBL
contact email	frank.stein@embl.de
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD023171
     1. Label: PRIDE project
     2. Name: Trait tacking for predictive evolution of metabolic pheotypes