PXD023168 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Proteome of paediatric burn wound fluid collected via negative pressure wound therapy |
Description | Negative pressure wound therapy (NPWT) has been used to promote wound healing in a variety of settings, including as an adjunct to silver-impregnated dressings in the acute management of paediatric burns. Fluid aspirated by the NPWT system represents a potentially insightful research matrix for understanding the burn wound microenvironment and the biochemical mechanisms of action of NPWT. The aim of this study was to characterise the proteome of wound fluid collected using NPWT from children with small-area thermal burns. Samples were obtained as part of a randomised controlled trial investigating the clinical efficacy of adjunctive NPWT. They were compared with blister fluid specimens from paediatric burn patients matched according to demographic and injury characteristics. Protein identification and quantification were performed via liquid chromatography tandem mass spectrometry (LC-MS/MS) and sequential window acquisition of all theoretical mass spectra (SWATH) data-independent acquisition. Proteins and biological pathways which were unique or enriched in negative pressure fluid samples were evaluated using principal components, partial least squares-discriminant, and gene ontology enrichment analyses. Eight viable samples of NPWT fluid were collected and analysed with eight matched blister fluid samples. A total of 502 proteins were quantitatively profiled in the NPWT fluid, of which 444 (88.4%) were shared with blister fluid. Several proteins exhibited significant abundance differences between fluid types, with NPWT fluid showing a higher abundance of proteases matrix metalloprotease-9 and arginase-1, immune cell surface molecule low affinity immunoglobulin gamma Fc region receptor III-A, actin-binding protein filamin-A, plasma protein alpha-2-macroglobulin, and haemoglobin subunit alpha. The results lend support to the hypothesis that NPWT augments wound healing through the modulation of factors involved in the inflammatory response, granulation tissue synthesis, and extracellular matrix maintenance. |
HostingRepository | PRIDE |
AnnounceDate | 2021-01-04 |
AnnouncementXML | Submission_2021-01-04_07:12:03.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Tuo Zang |
SpeciesList | scientific name: Homo sapiens (Human); NCBI TaxID: 9606; |
ModificationList | No PTMs are included in the dataset |
Instrument | TripleTOF 5600; TripleTOF 6600 |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2020-12-16 01:31:35 | ID requested | |
⏵ 1 | 2021-01-04 07:12:04 | announced | |
Publication List
Frear CC, Zang T, Griffin BR, McPhail SM, Parker TJ, Kimble RM, Cuttle L, The modulation of the burn wound environment by negative pressure wound therapy: Insights from the proteome. Wound Repair Regen, 29(2):288-297(2021) [pubmed] |
Keyword List
submitter keyword: NPWT, burn, paediatric, blister fluid, exudate, effluent, oedema fluid, proteomics, SWATH, LC-MS/MS |
Contact List
Leila Cuttle |
contact affiliation | Burns and Trauma Research,School of Biomedical Sciences, Queensland University of Technology |
contact email | leila.cuttle@qut.edu.au |
lab head | |
Tuo Zang |
contact affiliation | Queensland University of Technology |
contact email | tuo.zang@gmail.com |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD023168
- Label: PRIDE project
- Name: Proteome of paediatric burn wound fluid collected via negative pressure wound therapy