PXD023145

PXD023145 is an original dataset announced via ProteomeXchange.

Title	Deconstructing a type III secretion system effector network unravels the inherent robustness and plasticity in pathogenesis and immunity
Description	Infections with many Gram-negative pathogens rely on type III secretion system effectors. We hypothesized that while hijacking processes within mammalian cells, the effectors operate as a robust network which can tolerate significant contractions; this was tested in vivo using the mouse pathogen Citrobacter rodentium (encoding 31 effectors). Progressive gene deletions showed that effector essentiality for infection is context dependent and that the network can tolerate 60% contraction while maintaining pathogenicity. Despite inducing drastically different colonic cytokine profiles (e.g. IL-22, IL-17, IFN-γ or GM-CSF), different networks induced protective immunity. Using data from >100 distinct mutant combinations, we built and trained an in silico model able to predict colonization outcomes, which were confirmed experimentally. Furthermore, reproducing the human-restricted enteropathogenic E. coli effector repertoire in C. rodentium was not sufficient for efficient colonization, implicating effector networks in host adaptation. These results unveil the extreme robustness of both T3SS effector networks and host responses.
HostingRepository	PRIDE
AnnounceDate	2022-04-06
AnnouncementXML	Submission_2022-04-06_02:19:53.842.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	James Wright
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationList	TMT6plex-126 reporter+balance reagent acylated residue; monohydroxylated residue; iodoacetamide derivatized residue; deamidated residue
Instrument	Orbitrap Fusion Lumos; Orbitrap Fusion

Revision	Datetime	Status	ChangeLog Entry
0	2020-12-15 08:07:55	ID requested
⏵ 1	2022-04-06 02:19:54	announced

Dataset with its publication pending

submitter keyword: TMT, MS3, Mouse, Pathogen

Jyoti Choudhary
contact affiliation	The Institute of Cancer Research
contact email	jyoti.choudhary@icr.ac.uk
lab head
James Wright
contact affiliation	The Institute of Cancer Research
contact email	james.wright@icr.ac.uk
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD023145
     1. Label: PRIDE project
     2. Name: Deconstructing a type III secretion system effector network unravels the inherent robustness and plasticity in pathogenesis and immunity