PXD023144

PXD023144 is an original dataset announced via ProteomeXchange.

Title	Packed red blood cells inhibit T-cell activation via ROS-dependent signaling pathways
Description	Numerous observations indicate that red blood cells (RBCs) affect T-cell activation and proliferation. We have studied effects of packed RBCs (PRBCs) on T-cell-receptor (TCR) signaling and the molecular mechanisms whereby (P)RBCs modulate T-cell activation. In line with previous reports, PRBCs attenuated the expression of T-cell activation markers CD25 and CD69 upon co-stimulation via CD3/CD28. In addition, T-cell proliferation and cytokine expression were markedly reduced when T-cells were stimulated in presence of PRBCs. Inhibitory activity of PRBCs required direct cell-cell contact and intact PRBCs. The production of activation-induced cellular reactive oxygen species (ROS), which act as second messengers in T-cells, was completely abrogated to levels of unstimulated T-cells in presence of PRBCs. Phosphorylation of the TCR-related zeta-chain and thus proximal TCR signal transduction was unaffected by PRBCs, ruling out mechanisms based on secreted factors and steric interaction restrictions. Only downstream signaling events requiring ROS for full functionality were affected, confirmed by an untargeted mass spectrometry-based phosphoproteomics approach. PRBCs inhibited T-cell activation more efficiently than did treatment with 1 mM of the anti-oxidant N-acetyl cysteine. Taken together, our data suggest that inflammation-related radical reactions are modulated by PRBCs. These immunomodulating effects might be responsible for clinical observations associated with transfusion of PRBCs.
HostingRepository	PRIDE
AnnounceDate	2021-06-01
AnnouncementXML	Submission_2021-06-01_04:15:53.061.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Christopher Gerner
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	phosphorylated residue; acetylated residue; monohydroxylated residue; iodoacetamide derivatized residue
Instrument	timsTOF Pro

Revision	Datetime	Status	ChangeLog Entry
0	2020-12-15 08:07:53	ID requested
⏵ 1	2021-06-01 04:15:53	announced

Gerner MC, Bileck A, Janker L, Ziegler LS, Ö, hlinger T, Raeven P, M, ü, llner EW, Salzer U, Gerner C, Schmetterer KG, Baron DM, Packed red blood cells inhibit T-cell activation via ROS-dependent signaling pathways. J Biol Chem, 296():100487(2021) [pubmed]

submitter keyword: T‐cell, erythrocyte, reactive oxygen species (ROS), immunosuppressor, redox regulation, phosphoproteomics

Christopher Gerner
contact affiliation	University of Vienna, Department of Analytical Chemistry, Joint Metabolome Facility
contact email	christopher.gerner@univie.ac.at
lab head
Christopher Gerner
contact affiliation	University of Vienna
contact email	christopher.gerner@univie.ac.at
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD023144
     1. Label: PRIDE project
     2. Name: Packed red blood cells inhibit T-cell activation via ROS-dependent signaling pathways