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PXD023125

PXD023125 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleTranslatome proteomics of human AML cells treated with FLT3 inhibitors quizartinib, crenolanib, gilteritinib
DescriptionInternal tandem duplications (ITD) in the receptor tyrosine kinase FLT3 occur in 25% of acute myeloid leukemia (AML) patients and lead to constitutive activation of FLT3, driving leukemia cell survival and proliferation. Quizartinib, crenolanib and gilteritinib are second-generation FLT3 inhibitors (FLT3i) in phase III trials or clinical use for the targeted treatment of FLT3-ITD+ AML. However, they demonstrated only limited benefit and were not curative. A full understanding of cellular resistance factors contributing to this poor response is lacking. Here, we examined cell-autonomous pathways modulated by FLT3i using global translatome and phosphoproteome proteomics to identify non-genetic resistance mechanisms.
HostingRepositoryPRIDE
AnnounceDate2022-08-25
AnnouncementXMLSubmission_2022-08-25_04:44:53.418.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterSebastian Koschade
SpeciesList scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationListTMT6plex-126 reporter+balance reagent acylated residue; 6x(13)C labeled residue; iodoacetamide derivatized residue
InstrumentQ Exactive HF
Dataset History
RevisionDatetimeStatusChangeLog Entry
02020-12-14 07:20:15ID requested
12022-08-25 04:44:54announced
Publication List
Koschade SE, Klann K, Shaid S, Vick B, Stratmann JA, Th, ö, lken M, Meyer LM, Nguyen TD, Campe J, Moser LM, Hock S, Baker F, Meyer CT, Wempe F, Serve H, Ullrich E, Jeremias I, M, ü, nch C, Brandts CH, Translatome proteomics identifies autophagy as a resistance mechanism to on-target FLT3 inhibitors in acute myeloid leukemia. Leukemia, 36(10):2396-2407(2022) [pubmed]
Keyword List
submitter keyword: Human, AML, FLT3, LC-MSMS
Contact List
Christian Münch
contact affiliationInstitute of Biochemistry II, Faculty of Medicine, Goethe University, Frankfurt, Germany
contact emailch.muench@em.uni-frankfurt.de
lab head
Sebastian Koschade
contact affiliationDepartment of Medicine, Hematology/Oncology, University Hospital Frankfurt, Goethe University, Frankfurt, Germany & Institute of Biochemistry II, Faculty of Medicine, Goethe University, Frankfurt, Germany
contact emailkoschade@med.uni-frankfurt.de
dataset submitter
Full Dataset Link List
Dataset FTP location
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