PXD023121

PXD023121 is an original dataset announced via ProteomeXchange.

Title	FAM83F regulates canonical Wnt signalling through an interaction with CK1α
Description	The function of the FAM83F protein, like the functions of many members of the FAM83 family, is poorly understood. Here we show that injection of Fam83f mRNA into Xenopus embryos causes axis duplication, a phenotype indicative of enhanced Wnt signalling. Consistent with this, overexpression of FAM83F activates Wnt signalling, whilst ablation of FAM83F from human colorectal cancer (CRC) cells attenuates it. We demonstrate that FAM83F is farnesylated and interacts and co-localises with CK1α at the plasma membrane. This interaction with CK1α is essential for FAM83F to activate Wnt signalling, and FAM83F mutants that do not interact with CK1α fail to induce axis duplication in Xenopus embryos and to activate Wnt signalling in cells. FAM83F acts upstream of GSK-3β, because the attenuation of Wnt signalling caused by loss of FAM83F can be rescued by GSK-3 inhibition. Introduction of a farnesyl-deficient mutant of FAM83F in cells through CRISPR/Cas9 genome editing redirects the FAM83F-CK1α complex away from the plasma membrane and significantly attenuates Wnt signalling, indicating that FAM83F exerts its effects on Wnt signalling at the plasma membrane.
HostingRepository	PRIDE
AnnounceDate	2020-12-23
AnnouncementXML	Submission_2020-12-23_10:57:38.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Gopal Sapkota
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	farnesylated residue
Instrument	LTQ Orbitrap Velos

Revision	Datetime	Status	ChangeLog Entry
0	2020-12-14 06:44:45	ID requested
⏵ 1	2020-12-23 10:57:38	announced

Dataset with its publication pending

submitter keyword: FAM83F

Gopal Sapkota
contact affiliation	MRC-PPU
contact email	g.sapkota@dundee.ac.uk
lab head
Gopal Sapkota
contact affiliation	University of Dundee
contact email	g.sapkota@dundee.ac.uk
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD023121
     1. Label: PRIDE project
     2. Name: FAM83F regulates canonical Wnt signalling through an interaction with CK1α