PXD023108

PXD023108 is an original dataset announced via ProteomeXchange.

Title	Temporal proteomic changes induced by nicotine in human cells: A quantitative proteomics approach
Description	Nicotine is a prominent active compound in tobacco and many smoking cessation products. Some of the biological effects of nicotine are well documented in in vitro and in vivo systems; however, nominal data are available concerning the time-dependent changes on protein and phosphorylation events in response to nicotine. Here, we profiled the proteomes of SH-SY5Y and A549 cell lines subjected to acute (15min, 1h and 4h) or chronic (24h, 48h) nicotine exposures. We used sample multiplexing (TMTpro16) and quantified more than 9,000 proteins and over 7,000 phosphorylation events per cell line. Among our findings, we determined a decrease in mitochondrial protein abundance for SH-SY5Y, while we detected alterations in several immune pathways, such as the complement system, for A549. We also explored the proposed association between smoking and SARS-CoV2. Here, we found several host proteins known to interact with viral proteins that were affected by nicotine in a time dependent manner. This dataset can be mined further to investigate the potential role of nicotine in different biological contexts.
HostingRepository	PRIDE
AnnounceDate	2021-05-11
AnnouncementXML	Submission_2021-05-11_06:21:21.097.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Joao Paulo
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Orbitrap Fusion Lumos

Revision	Datetime	Status	ChangeLog Entry
0	2020-12-14 01:10:57	ID requested
⏵ 1	2021-05-11 06:21:22	announced

Navarrete-Perea J, Gygi SP, Paulo JA, Temporal proteomic changes induced by nicotine in human cells: A quantitative proteomics approach. J Proteomics, 241():104244(2021) [pubmed]

submitter keyword: COVID19, FAIMS, Orbitrap Lumos, Protein-protein interactions, Real Time Search, RTS, TMTpro

Joao A. Paulo
contact affiliation	Harvard Medical School, Boston, MA
contact email	joao_paulo@post.hms.harvard.edu
lab head
Joao Paulo
contact affiliation	Harvard Medical School
contact email	joao_paulo@post.harvard.edu
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD023108
     1. Label: PRIDE project
     2. Name: Temporal proteomic changes induced by nicotine in human cells: A quantitative proteomics approach