PXD023106

PXD023106 is an original dataset announced via ProteomeXchange.

Title	ID3 Promotes Homologous Recombination via Non-transcriptional and Transcriptional Mechanisms and its loss confers Sensitivity to PARP
Description	The inhibitor of DNA-binding 3 (ID3) is a transcriptional regulator that limits interaction of basic helix-loop-helix transcription factors with the DNA. ID3 expression is frequently deregulated in human cancers and its loss was shown to impact DNA repair. To understand the molecular mechanisms by which ID3 regulates DNA repair, proteomic and transcriptomic approaches following DNA damage induction were performed in wild-type and ID3-depleted cells. Results show that ID3 promotes DNA double-strand break repair, particularly homologous recombination (HR). Mechanistically, two main pathways were identified through which ID3 contributes to HR, (1) via protein interaction with RAD50 and RECQL, and (2) as a transcriptional regulator. In the latter case, ID3 is required for the expression of specific HR genes in response to ionizing radiation. We conclude that loss of ID3 leads to HR deficiency which subsequently confers sensitivity to PARP inhibition, thus offering new therapeutic options to ID3 deficient cancers.
HostingRepository	PRIDE
AnnounceDate	2025-04-08
AnnouncementXML	Submission_2025-04-08_03:37:38.166.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Gianluca Sigismondo
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	No PTMs are included in the dataset
Instrument	Orbitrap Fusion

Revision	Datetime	Status	ChangeLog Entry
0	2020-12-14 01:10:56	ID requested
⏵ 1	2025-04-08 03:37:39	announced

Bakr A, Hey J, Sigismondo G, Liu CS, Sadik A, Goyal A, Cross A, Iyer RL, M, ü, ller P, Trauernicht M, Breuer K, Lutsik P, Opitz CA, Krijgsveld J, Weichenhan D, Plass C, Popanda O, Schmezer P, ID3 promotes homologous recombination via non-transcriptional and transcriptional mechanisms and its loss confers sensitivity to PARP inhibition. Nucleic Acids Res, 49(20):11666-11689(2021) [pubmed]

10.1093/nar/gkab964;

submitter keyword: cancer,ID3, HDR, PARPi, DSB

Peter Schmezer
contact affiliation	Division of Cancer Epigenomics, German Cancer Research Center (DKFZ), INF280, 69120 Heidelberg, Germany.
contact email	p.schmezer@dkfz-heidelberg.de
lab head
Gianluca Sigismondo
contact affiliation	DKFZ
contact email	gianluca.sigismondo@gmail.com
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2025/04/PXD023106

PRIDE project URI

• PRIDE
  1. PXD023106
     1. Label: PRIDE project
     2. Name: ID3 Promotes Homologous Recombination via Non-transcriptional and Transcriptional Mechanisms and its loss confers Sensitivity to PARP