PXD023106 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | ID3 Promotes Homologous Recombination via Non-transcriptional and Transcriptional Mechanisms and its loss confers Sensitivity to PARP |
Description | The inhibitor of DNA-binding 3 (ID3) is a transcriptional regulator that limits interaction of basic helix-loop-helix transcription factors with the DNA. ID3 expression is frequently deregulated in human cancers and its loss was shown to impact DNA repair. To understand the molecular mechanisms by which ID3 regulates DNA repair, proteomic and transcriptomic approaches following DNA damage induction were performed in wild-type and ID3-depleted cells. Results show that ID3 promotes DNA double-strand break repair, particularly homologous recombination (HR). Mechanistically, two main pathways were identified through which ID3 contributes to HR, (1) via protein interaction with RAD50 and RECQL, and (2) as a transcriptional regulator. In the latter case, ID3 is required for the expression of specific HR genes in response to ionizing radiation. We conclude that loss of ID3 leads to HR deficiency which subsequently confers sensitivity to PARP inhibition, thus offering new therapeutic options to ID3 deficient cancers. |
HostingRepository | PRIDE |
AnnounceDate | 2025-04-08 |
AnnouncementXML | Submission_2025-04-08_03:37:38.166.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Gianluca Sigismondo |
SpeciesList | scientific name: Homo sapiens (Human); NCBI TaxID: 9606; |
ModificationList | No PTMs are included in the dataset |
Instrument | Orbitrap Fusion |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2020-12-14 01:10:56 | ID requested | |
⏵ 1 | 2025-04-08 03:37:39 | announced | |
Publication List
Bakr A, Hey J, Sigismondo G, Liu CS, Sadik A, Goyal A, Cross A, Iyer RL, M, ΓΌ, ller P, Trauernicht M, Breuer K, Lutsik P, Opitz CA, Krijgsveld J, Weichenhan D, Plass C, Popanda O, Schmezer P, ID3 promotes homologous recombination via non-transcriptional and transcriptional mechanisms and its loss confers sensitivity to PARP inhibition. Nucleic Acids Res, 49(20):11666-11689(2021) [pubmed] |
10.1093/nar/gkab964; |
Keyword List
submitter keyword: cancer,ID3, HDR, PARPi, DSB |
Contact List
Peter Schmezer |
contact affiliation | Division of Cancer Epigenomics, German Cancer Research Center (DKFZ), INF280, 69120 Heidelberg, Germany. |
contact email | p.schmezer@dkfz-heidelberg.de |
lab head | |
Gianluca Sigismondo |
contact affiliation | DKFZ |
contact email | gianluca.sigismondo@gmail.com |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD023106
- Label: PRIDE project
- Name: ID3 Promotes Homologous Recombination via Non-transcriptional and Transcriptional Mechanisms and its loss confers Sensitivity to PARP