PXD023100

PXD023100 is an original dataset announced via ProteomeXchange.

Title	GwAAP: A Genome-wide Amino Acid coding-decoding quantitative Proteomic system
Description	GwAAP: A Genome-wide Amino Acid coding-decoding quantitative Proteomic system was designed in which each protein could be assigned with a distinct code. Using synthetic biology technology, the amino acids codes were coded in N terminal of the corresponding proteins in the genome of yeast (Saccharomyces cerevisiae), so that the copy number of the code sequences were identical with the targeted proteins in yeast. The code sequences were enriched by HA antibody and decoded and quantified by mass spectrometry strategy. In general, this method increased the detection sensitivity by peptide enrichment, which reduced the dynamic range and the complexity of proteomics. In addition, coding sequence with similar physical and chemical properties resulted in the comparable linear signal response of the mass spectrum, which significantly improve the accuracy of proteomic quantification.
HostingRepository	iProX
AnnounceDate	2021-12-22
AnnouncementXML	Submission_2023-09-11_20:59:17.064.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Xuetong Yue
SpeciesList	scientific name: Saccharomyces cerevisiae; NCBI TaxID: 4932;
ModificationList	No PTMs are included in the dataset
Instrument	Q Exactive HF; Q Exactive HF-X

Revision	Datetime	Status	ChangeLog Entry
0	2020-12-13 18:00:36	ID requested
⏵ 1	2023-09-11 20:59:18	announced

Cheng L, Yue X, Qin Z, Sun X, He F, Dai J, Ding C, GwAAP: A genome-wide amino acid coding-decoding quantitative proteomics system. iScience, 25(12):105471(2022) [pubmed]

submitter keyword: Amino acid codes, synthetic biology, proteomics, protein quantification

Fuchu He
contact affiliation	Fudan University
contact email	hefc@nic.bmi.ac.cn
lab head
Xuetong Yue
contact affiliation	Fudan University
contact email	18111510021@fudan.edu.cn
dataset submitter

iProX dataset URI