PXD023088

PXD023088 is an original dataset announced via ProteomeXchange.

Title	Nucleolin is a cell surface receptor of C1QTNF4
Description	C1QTNF4 is a secreted protein that is structurally unique within the C1QTNF family, a family of proteins that have structural homology with both complement C1q and the tumor necrosis factor superfamily. The role of C1QTNF4 in immunity and inflammation remains poorly defined and a cell surface receptor has yet to be identified. Here we performed co-immunoprecipitation with subsequent mass spectrometric analysis in order to identify a cell surface receptor of C1QTNF4. We report identification of nucleolin as a cell surface binding partner of C1QTNF4. Nucleolin may serve as a docking molecule for C1QTNF4 and act in a context-dependent manner through co-receptors. Having identified nucleolin as a cell surface receptor is a further step to understanding C1QTNF4’s function in the healthy immune system.
HostingRepository	PRIDE
AnnounceDate	2021-03-22
AnnouncementXML	Submission_2021-03-21_23:07:12.718.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Susan Vester
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Orbitrap Fusion Lumos

Revision	Datetime	Status	ChangeLog Entry
0	2020-12-13 11:08:28	ID requested
1	2021-03-21 14:09:37	announced
⏵ 2	2021-03-21 23:07:13	announced	2021-03-22: Updated project metadata.

Vester SK, Beavil RL, Lynham S, Beavil AJ, Cunninghame Graham DS, McDonnell JM, Vyse TJ, Nucleolin acts as the receptor for C1QTNF4 and supports C1QTNF4-mediated innate immunity modulation. J Biol Chem, 296():100513(2021) [pubmed]

10.1016/J.JBC.2021.100513;

submitter keyword: innate immunity, nucleolin, cell surface receptor, inflammation, CTRP4, C1QTNF4, monocyte,autoimmune disease, systemic lupus erythematosus

Timothy J Vyse
contact affiliation	Department of Medical & Molecular Genetics, King’s College London, United Kingdom
contact email	timothy.vyse@kcl.ac.uk
lab head
Susan Vester
contact affiliation	King's College London
contact email	susan.vester@kcl.ac.uk
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD023088
     1. Label: PRIDE project
     2. Name: Nucleolin is a cell surface receptor of C1QTNF4