PXD023084

PXD023084 is an original dataset announced via ProteomeXchange.

Title	High intensity interval training remodels the proteome and acetylome in human skeletal muscle
Description	Exercise is an effective strategy in the prevention and treatment of metabolic diseases. Alterations in the skeletal muscle proteome, including post-translational modifications, regulate its metabolic adaptations to exercise. Here, we examined the effect of high-intensity interval training (HIIT) on the proteome and acetylome of human skeletal muscle, revealing the response of 3168 proteins and 1263 lysine acetyl-sites on 464 acetylated proteins. We identified novel protein adaptations to exercise training involved in metabolism and excitation-contraction coupling. Furthermore, HIIT increased the acetylation of mitochondrial proteins, particularly those of complex V, likely via non-enzymatic mechanisms. We also highlight the regulation of novel exercise-responsive histone acetyl-sites. These data demonstrate the plasticity of the skeletal muscle proteome and acetylome, providing insight into the regulation of contractile, metabolic and transcriptional processes within skeletal muscle. Herein, we provide a substantial hypothesis-generating resource to stimulate further mechanistic research investigating how exercise improves metabolic health.
HostingRepository	PRIDE
AnnounceDate	2022-06-09
AnnouncementXML	Submission_2022-06-09_04:34:13.558.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	atul shahaji deshmukh
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	acetylated residue
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2020-12-12 11:38:35	ID requested
⏵ 1	2022-06-09 04:34:14	announced

Hostrup M, Lemminger AK, Stocks B, Gonzalez-Franquesa A, Larsen JK, Quesada JP, Thomassen M, Weinert BT, Bangsbo J, Deshmukh AS, High-intensity interval training remodels the proteome and acetylome of human skeletal muscle. Elife, 11():(2022) [pubmed]

submitter keyword: Exercise, skeletal muscle, LCMS, Proteome, lysine acetylation

Atul Shahaji Deshmukh
contact affiliation	Novo Nordisk Center For Basic Metabolic Research, Functional Proteomics in Metabolism Group, Department of Health and Medical Science. University of Copenhagen, Denmark.
contact email	atul.deshmukh@sund.ku.dk
lab head
atul shahaji deshmukh
contact affiliation	Copenhagen University
contact email	atul.deshmukh@cpr.ku.dk
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD023084
     1. Label: PRIDE project
     2. Name: High intensity interval training remodels the proteome and acetylome in human skeletal muscle