PXD023082

PXD023082 is an original dataset announced via ProteomeXchange.

Title	Immunoprecipitation-Mass Spectrometry (IP-MS) identifies components of a Mce4A-containing complex
Description	How lipids travel between membranes of diderm bacteria is a challenging mechanistic question because lipids, which are hydrophobic molecules, must traverse a hydrophilic periplasm. This question is even more complex for mycobacteria, which have a unique cell envelope that is highly impermeable to molecules. A growing body of knowledge identifies Mce transporters as lipids importers for mycobacteria. Here, using protein stability experiments and immunoprecipitation-mass spectrometry to identify protein interactions among components of the mycobacterial Mce4 transporter we provide evidence for Mce transporters existing as multiprotein complexes.
HostingRepository	PRIDE
AnnounceDate	2022-02-16
AnnouncementXML	Submission_2022-02-16_10:31:11.996.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Laura Herring
SpeciesList	scientific name: Mycolicibacterium smegmatis MC2 155; NCBI TaxID: 246196;
ModificationList	No PTMs are included in the dataset
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2020-12-12 11:38:03	ID requested
⏵ 1	2022-02-16 10:31:13	announced

Rank L, Herring LE, Braunstein M, Evidence for the Mycobacterial Mce4 Transporter Being a Multiprotein Complex. J Bacteriol, 203(10):(2021) [pubmed]

submitter keyword: AP-MS, IP-MS, Mce transporters, mycobacteria

Miriam Braunstein
contact affiliation	Department of Microbiology and Immunology, UNC-Chapel Hill
contact email	miriam_braunstein@med.unc.edu
lab head
Laura Herring
contact affiliation	UNC-Chapel Hill
contact email	laura_herring@med.unc.edu
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2022/02/PXD023082

PRIDE project URI

• PRIDE
  1. PXD023082
     1. Label: PRIDE project
     2. Name: Immunoprecipitation-Mass Spectrometry (IP-MS) identifies components of a Mce4A-containing complex