PXD023080

PXD023080 is an original dataset announced via ProteomeXchange.

Title	A novel exopolysaccharide EPS364 from Vibrio alginolyticus inhibit cancer cell growth via blocking cell adhesion
Description	The prognosis of liver cancer was inferior among tumors. New medicine treatments are urgently needed. In this study, a novel exopolysaccharide EPS364 was purified from Vibrio alginolyticus 364 which was isolated from South China Sea cold seep. Further research suggested that EPS364 consisted of mannose, glucosamine, gluconic acid, galactosamine, arabinose with a molar ratio of 5:9:3.4:0.8:1.5. The molecular weight of EPS364 was 14.8 kDa. Our results further indicated that EPS364 was β-linked and phosphorylated polysaccharide. Notably, EPS364 exhibited significant anti-tumor activity. Besides, EPS364 induced Huh7.5 cells apoptosis, collapse of mitochondrial membrane potential (MMP) and generation of reactive oxygen species (ROS). Proteomic and quantitative real-time PCR analyses indicated that EPS364 blocked cancer cell adhesion and induced apoptosis via targeting FGF19–FGFR4 signaling pathway. These findings suggested that EPS364 was a promising anti-tumor agent for pharmacotherapy.
HostingRepository	PRIDE
AnnounceDate	2021-09-09
AnnouncementXML	Submission_2021-09-09_15:00:18.604.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Yun Wang
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	No PTMs are included in the dataset
Instrument	Q Exactive Plus

Revision	Datetime	Status	ChangeLog Entry
0	2020-12-12 11:37:32	ID requested
⏵ 1	2021-09-09 15:00:19	announced

Wang Y, Liu G, Liu R, Wei M, Zhang J, Sun C, EPS364, a Novel Deep-Sea Bacterial Exopolysaccharide, Inhibits Liver Cancer Cell Growth and Adhesion. Mar Drugs, 19(3):(2021) [pubmed]

submitter keyword: Exopolysaccharide

anti-tumor

FGF19–FGFR4 signaling

cell adhesion

Chaomin Sun
contact affiliation	CAS Key Laboratory of Experimental Marine Biology & Center of Deep Sea Research, Institute of Oceanology, Chinese Academy of Sciences, Qingdao, 266071, China
contact email	sunchaomin@qdio.ac.cn
lab head
Yun Wang
contact affiliation	Key Laboratory of Experimental Marine Biology, Institute of Oceanology, Chinese Academy of Sciences, Qingdao, China
contact email	wangyunxixi@yeah.net
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2021/09/PXD023080

PRIDE project URI

• PRIDE
  1. PXD023080
     1. Label: PRIDE project
     2. Name: A novel exopolysaccharide EPS364 from Vibrio alginolyticus inhibit cancer cell growth via blocking cell adhesion