PXD023076

PXD023076 is an original dataset announced via ProteomeXchange.

Title	Omental adipose tissue metaboproteome: differential dysregulation by chronic binge alcohol and ovariectomy in simian immunodeficiency virus-infected female macaques
Description	Effective antiretroviral therapy (ART) has significantly reduced mortality of people living with HIV (PLWH). Increased survival and aging of PLWH is associated with increased incidence of at-risk alcohol use and of metabolic comorbidities; and in women, menopause may be an additional factor contributing to metabolic dysregulation, particularly in adipose tissue. We examined the differential effects of chronic binge alcohol (CBA) administration and ovariectomy (OVX) on the omental adipose tissue (OmAT) proteome of simian immunodeficiency virus (SIV) infected macaques. Quantitative discovery-based proteomics identified 1429 differentially expressed proteins. Ingenuity Pathway Analysis calculated z-scores, or activation predictions for a disease or functional pathway. Results revealed functional pathways associated with protein changes centered around the “OmAT metaboproteome profile”. CBA did not affect functional pathways of metabolic disease; but dysregulated proteins involved in AMPK signaling and lipid metabolism. Based on IPA’s calculated z-score, OVX-mediated proteome changes promote pathways associated with glucose and lipid associated metabolic disease. Additionally, OVX was predicted to promote apoptosis, necrosis and reactive oxygen species (ROS) pathways, while CBA was predicted to inhibit these OVX-associated changes. These results provide evidence for the role of ovarian hormone loss in mediating OmAT metaboproteome dysregulation in HIV/SIV and suggest that CBA modifies OVX-associated changes. In the context of OVX, CBA administraton produced larger metabolic and cellular effects; which we speculate may reflect a protective role of estrogen against CBA-mediated adipose tissue injury in female SIV-infected macaques.
HostingRepository	PRIDE
AnnounceDate	2022-08-12
AnnouncementXML	Submission_2022-08-12_00:19:34.863.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD023076
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Jessie Guidry
SpeciesList	scientific name: Macaca mulatta (Rhesus macaque); NCBI TaxID: 9544;
ModificationList	TMT6plex-126 reporter+balance reagent acylated residue
Instrument	Orbitrap Fusion

Revision	Datetime	Status	ChangeLog Entry
0	2020-12-11 08:44:07	ID requested
⏵ 1	2022-08-12 00:19:35	announced

Poret JM, Guidry JJ, Simon L, Molina PE, Chronic binge alcohol and ovariectomy dysregulate omental adipose tissue metaboproteome in simian immunodeficiency virus-infected female macaques. Physiol Genomics, 53(8):358-371(2021) [pubmed]

submitter keyword: adipose alcohol ovariectomy SIV macaques LC-MS TMTpro

Jessie Guidry
contact affiliation	Department of Biochemistry and Molecular Biology Proteomics Core Facility LSUHSC New Orleans, LA 70112
contact email	jjguid@lsuhsc.edu
lab head
Jessie Guidry
contact affiliation	Louisiana State University Health Sciences Center Department of Biochemistry and Molecular Biology LSUHSC Proteomics Core Facility
contact email	jjguid@lsuhsc.edu
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD023076
     1. Label: PRIDE project
     2. Name: Omental adipose tissue metaboproteome: differential dysregulation by chronic binge alcohol and ovariectomy in simian immunodeficiency virus-infected female macaques