PXD023063

PXD023063 is an original dataset announced via ProteomeXchange.

Title	Comparative proteomic analysis of secretomes from engineered Penicillium funiculosum modulated with sugarcane bagasse
Description	Sugarcane bagasse (SCB), one of the most abundant plant feedstocks is at the leading front of the biofuel industry based on the potential to promote economic, social and environmental development worldwide through sustainable set-ups related to energy production. This complex biomass requires a vast array of carbohydrate active enzymes (CAZymes) mostly from filamentous fungi for its deconstruction to monomeric sugars for the production of value-added fuels and chemicals. In this study, we attempted to evaluate the comprehensive repertoire of proteins in the secretome of an engineered Penicillium funiculosum, (PfMig188) in response to SCB. For this, a systematic approach was utilized for the cultivation of the fungus towards production of tailored enzymes specific for saccharification of SCB. This was done through the modulation of the production media with different concentrations of pretreated SCB (0 – 45 g/L) and the array of secreted proteins were identified by enzyme activity assay and liquid chromatography-tandem mass spectrometry (LC-MS/MS). A total of 280 proteins were detected in the secretome of PfMig188 with 46% of them being CAZymes. Modulation of the cultivation media with SCB up till 15 g/L enhanced the secretion of some importanthemicellulasesand cell wall modifying enzymes such as β-xylosidase (GH5, GH43), β-1,3-galactosidase (GH43), endo-1,3(4)-beta-glucanase (GH16), cutinase (CE5) and hydrophobic surface binding protein (HsbA)that works in synergy with the cellulases in the secretome to improve SCB saccharification by 20%. Our findings provide more insight on the enzyme system of PfMig188 for degradation of complex biomass such as SCB and highlights the important role of adjusting the culture medium composition with SCB for secretion of enzymes specific for its saccharification.
HostingRepository	PRIDE
AnnounceDate	2021-09-09
AnnouncementXML	Submission_2021-09-09_15:04:20.203.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD023063
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Olusola Ogunyewo
SpeciesList	scientific name: Penicillium sp. B12; NCBI TaxID: 524867;
ModificationList	No PTMs are included in the dataset
Instrument	Orbitrap Fusion Lumos

Revision	Datetime	Status	ChangeLog Entry
0	2020-12-11 04:34:50	ID requested
⏵ 1	2021-09-09 15:04:20	announced

Ogunyewo OA, Upadhyay P, Rajacharya GH, Okereke OE, Faas L, G, ó, mez LD, McQueen-Mason SJ, Yazdani SS, Accessory enzymes of hypercellulolytic Penicillium funiculosum facilitate complete saccharification of sugarcane bagasse. Biotechnol Biofuels, 14(1):171(2021) [pubmed]

submitter keyword: Sugarcane bagasse, Proteomics, Engineered PfMig188, CAZymes, Biomass saccharification, quantitative mass spectrometry

Syed Shams Yazdani
contact affiliation	ICGEB
contact email	shams@icgeb.res.in
lab head
Olusola Ogunyewo
contact affiliation	Microbial Engineering Group, International Centre for Genetic engineering and Biotechnology, New Delhi, India
contact email	akintayo@icgeb.res.in
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD023063
     1. Label: PRIDE project
     2. Name: Comparative proteomic analysis of secretomes from engineered Penicillium funiculosum modulated with sugarcane bagasse