PXD023037

PXD023037 is an original dataset announced via ProteomeXchange.

Title	Proteome profile of myelin in the zebrafish brain
Description	While the myelin proteome is well characterized in mice, much less is known about the protein composition of myelin in non-mammalian species such as zebrafish (Danio rerio), despite its increasing popularity as model organism for myelin biology. Here, we assess the proteome of myelin biochemically purified from the brains of adult zebrafish by complementary proteomic approaches for deep qualitative and quantitative coverage. A data-independent acquisition (DIA) workflow with alternating low and elevated energy (MSE) provided the high dynamic range required for correct quantification of highest-abundance proteins. According to this analysis, the predominant Ig-CAM myelin protein zero (MPZ/P0), myelin basic protein (MBP) and the short chain dehydrogenase 36K constitute 12%, 8% and 6% of the total myelin protein, respectively. Combined with data from an ion mobility-enhanced version of the MSE mode (referred to as UDMSE) and from 1D-gel-based proteomic approaches, we provide the most comprehensive proteome resource of zebrafish myelin so far, correlate it with previously established mRNA-abundance profiles, and demonstrate both similarities and heterogeneity of myelin composition between teleost fish and rodents.
HostingRepository	PRIDE
AnnounceDate	2021-03-22
AnnouncementXML	Submission_2022-03-02_06:54:10.678.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Olaf Jahn
SpeciesList	scientific name: Danio rerio (Zebrafish) (Brachydanio rerio); NCBI TaxID: 7955;
ModificationList	No PTMs are included in the dataset
Instrument	Synapt MS

Revision	Datetime	Status	ChangeLog Entry
0	2020-12-10 00:50:05	ID requested
1	2021-03-22 03:36:50	announced
⏵ 2	2022-03-02 06:54:11	announced	2022-03-02: Updated publication reference for PubMed record(s): 33898427.

Siems SB, Jahn O, Hoodless LJ, Jung RB, Hesse D, M, ö, bius W, Czopka T, Werner HB, Proteome Profile of Myelin in the Zebrafish Brain. Front Cell Dev Biol, 9():640169(2021) [pubmed]

submitter keyword: Oligodendrocyte, myelin proteome, central nervous system (CNS), label-free proteomics, transcriptome, PLP, MBP, MPZ, CD59, teleost, zebrafish, danio rerio

Olaf Jahn
contact affiliation	Proteomics Group Max-Planck-Institute of Experimental Medicine Hermann-Rein-Str. 3 37075 Goettingen, Germany
contact email	jahn@em.mpg.de
lab head
Olaf Jahn
contact affiliation	Proteomics Group, Max-Planck-Institute of Experimental Medicine, Göttingen
contact email	jahn@em.mpg.de
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2021/03/PXD023037

PRIDE project URI

• PRIDE
  1. PXD023037
     1. Label: PRIDE project
     2. Name: Proteome profile of myelin in the zebrafish brain