PXD023020
PXD023020 is an original dataset announced via ProteomeXchange.
Dataset Summary
| Title | Co-immunoprecipitated human proteins with tobacco NtFT4 |
| Description | Biopharmaceutical proteins are produced in mammalian cells by transient expression or stable transformation, but both processes require robust and viable cells. Cell line engineering must therefore balance improved cell growth and viability with high productivity. We tested the expression of non-mammalian phosphatidylethanolamine binding proteins to enhance cell proliferation. The tobacco protein NtFT4 improved the proliferation and viability of HEK-293T cells in animal component-free suspension cultures. Viable cell density is usually impaired by efficient transfection, but we found that the number of HEK-293TNtFT4 cells doubled at the peak of protein expression and the antibody yield increased by approximately one third. The improved growth and viability were observed in different cell backgrounds, suggesting the system is transferable. Furthermore, high densities of viable HEK-293TNtFT4 cells will allow further modifications to boost protein synthesis. The established HEK-293TNtFT4 cell line provides a new human cell platform that increases cell proliferation without sacrificing translational activity. |
| HostingRepository | jPOST |
| AnnounceDate | 2021-12-10 |
| AnnouncementXML | Submission_2023-02-07_00:00:01.858.xml |
| DigitalObjectIdentifier | |
| ReviewLevel | Peer-reviewed dataset |
| DatasetOrigin | Original dataset |
| RepositorySupport | Unsupported dataset by repository |
| PrimarySubmitter | Jürgen Eirich |
| SpeciesList | scientific name: Homo sapiens (Human); NCBI TaxID: 9606; |
| ModificationList | S-carboxamidomethyl-L-cysteine; N6-acetyl-L-lysine; L-methionine sulfoxide |
| Instrument | Q Exactive |
Dataset History
| Revision | Datetime | Status | ChangeLog Entry |
|---|---|---|---|
| 0 | 2020-12-09 08:15:42 | ID requested | |
| 1 | 2021-12-09 07:00:05 | announced | |
| 2 | 2022-09-18 03:13:05 | announced | 2022-09-18: Updated FTP location. |
| ⏵ 3 | 2023-02-07 00:00:02 | announced | 2023-02-07: Updated PubMed. |
Publication List
| K, ä, nel P, Noll GA, Schroedter K, Naffin E, Kronenberg J, Busswinkel F, Twyman RM, Kl, ä, mbt C, Pr, ü, fer D, by interacting with the proteostasis network. Aging (Albany NY), 14(7):2989-3029(2022) [pubmed] |
Keyword List
| submitter keyword: phosphatidylethanolamine binding protein, proliferation, cell adhesion, insulin response, IP, co-ip, HEK-293T, tabacco, NtFT4 |
Contact List
| Philip Kaenel | |
|---|---|
| lab head | |
| Jürgen Eirich | |
| contact affiliation | University of Münster - WWU - Institute for Biology and Biotechnology of Plants |
| dataset submitter | |
Full Dataset Link List
| jPOST dataset URI |
| Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.jpostdb.org/JPST001031/ |
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