PXD023005

PXD023005 is an original dataset announced via ProteomeXchange.

Title	Metabolic control of inflammation by selenium
Description	Trace element selenium (Se) is incorporated as the 21st amino acid, selenocysteine (Sec), into selenoproteins through tRNA[Ser]Sec. Selenoproteins act as gatekeepers of redox homeostasis and modulate immune function to effect anti-inflammation and resolution. However, mechanistic underpinnings involving metabolic reprogramming during inflammation and resolution remain poorly understood. Endotoxin lipopolysaccharide (LPS) activation of murine bone marrow-derived macrophages (BMDMs) cultured in the presence or absence of Se (as selenite) was used to examine temporal changes in the proteome and metabolome by multiplexed tandem mass tag-quantitative proteomics, metabolomics, and machine-learning approaches. Se-dependent modulation of glycolytic, TCA and PPP pathways predisposed BMDMs to preferentially increase OXPHOS to efficiently regulate inflammation and its timely resolution. Use of macrophages lacking selenoproteins, indicated that all three metabolic nodes were sensitive to selenoproteome expression. Furthermore, inhibition of SDH with dimethylmalonate affected the pro-resolving effects of Se by increasing the resolution interval in a murine peritonitis model.
HostingRepository	PRIDE
AnnounceDate	2021-02-09
AnnouncementXML	Submission_2021-02-09_13:12:24.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	K. Sandeep Prabhu
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationList	No PTMs are included in the dataset
Instrument	Orbitrap Fusion

Revision	Datetime	Status	ChangeLog Entry
0	2020-12-09 00:18:53	ID requested
⏵ 1	2021-02-09 13:12:25	announced

Dataset with its publication pending

submitter keyword: Selenium, macrophages, proteomics, succinate dehydrogenase

K. Sandeep Prabhu
contact affiliation	Department of Veterinary and Biomedical Sciences, The Pennsylvania State University, University Park, PA 16802, USA
contact email	ksp4@psu.edu
lab head
K. Sandeep Prabhu
contact affiliation	Penn State University
contact email	ksp4@psu.edu
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2021/02/PXD023005

PRIDE project URI

• PRIDE
  1. PXD023005
     1. Label: PRIDE project
     2. Name: Metabolic control of inflammation by selenium